Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5587 | 5' | -58 | NC_001806.1 | + | 66809 | 0.66 | 0.790522 |
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Target: 5'- aUCacgGcCGAGCGggGCCgGCCCGGGuGGc -3' miRNA: 3'- cAGa--C-GCUCGUuaUGGaCGGGCCC-CU- -5' |
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| 5587 | 5' | -58 | NC_001806.1 | + | 65448 | 0.66 | 0.817028 |
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Target: 5'- ---aGCGGGgGAUGCCgcgGCCCccggguccuGGGGGc -3' miRNA: 3'- cagaCGCUCgUUAUGGa--CGGG---------CCCCU- -5' |
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| 5587 | 5' | -58 | NC_001806.1 | + | 9166 | 0.66 | 0.817028 |
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Target: 5'- aUCgGgGAGaCAAcgGCCguguaGCCCGGGGGu -3' miRNA: 3'- cAGaCgCUC-GUUa-UGGa----CGGGCCCCU- -5' |
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| 5587 | 5' | -58 | NC_001806.1 | + | 38557 | 0.66 | 0.825542 |
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Target: 5'- ---aGCGAGCG--ACCggGCCCgaaucGGGGAg -3' miRNA: 3'- cagaCGCUCGUuaUGGa-CGGG-----CCCCU- -5' |
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| 5587 | 5' | -58 | NC_001806.1 | + | 74880 | 0.66 | 0.825542 |
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Target: 5'- -cCUGCGGGCcuc-CCUGCCgGcGGGc -3' miRNA: 3'- caGACGCUCGuuauGGACGGgC-CCCu -5' |
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| 5587 | 5' | -58 | NC_001806.1 | + | 126473 | 0.66 | 0.833882 |
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Target: 5'- ---aGCGuGCGAUACUUcgcGCCCGGGc- -3' miRNA: 3'- cagaCGCuCGUUAUGGA---CGGGCCCcu -5' |
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| 5587 | 5' | -58 | NC_001806.1 | + | 144777 | 0.66 | 0.833882 |
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Target: 5'- cGUCUGUccccucacgGGGCGAagGCCgcguacgGCCCGGGa- -3' miRNA: 3'- -CAGACG---------CUCGUUa-UGGa------CGGGCCCcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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