Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5594 | 3' | -55.1 | NC_001806.1 | + | 117205 | 1.1 | 0.00303 |
Target: 5'- uACUGGUGCCGGUCGUGAGAGAAUCCCc -3' miRNA: 3'- -UGACCACGGCCAGCACUCUCUUAGGG- -5' |
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5594 | 3' | -55.1 | NC_001806.1 | + | 63595 | 0.68 | 0.88443 |
Target: 5'- uGCUGGUGucCCGGUCGaac-AGggUCCa -3' miRNA: 3'- -UGACCAC--GGCCAGCacucUCuuAGGg -5' |
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5594 | 3' | -55.1 | NC_001806.1 | + | 54513 | 0.68 | 0.88443 |
Target: 5'- gGCUGGUGaCUGc-CGUGGuguucuagaacGGGAAUCCCg -3' miRNA: 3'- -UGACCAC-GGCcaGCACU-----------CUCUUAGGG- -5' |
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5594 | 3' | -55.1 | NC_001806.1 | + | 115564 | 0.67 | 0.897856 |
Target: 5'- --gGGUGCCGcGaacUCGUG-GAGGA-CCCg -3' miRNA: 3'- ugaCCACGGC-C---AGCACuCUCUUaGGG- -5' |
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5594 | 3' | -55.1 | NC_001806.1 | + | 82141 | 0.67 | 0.910358 |
Target: 5'- -aUGGUGuCCaGGUCGccGAGGGAcagCCCc -3' miRNA: 3'- ugACCAC-GG-CCAGCa-CUCUCUua-GGG- -5' |
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5594 | 3' | -55.1 | NC_001806.1 | + | 49398 | 0.67 | 0.916256 |
Target: 5'- --cGGUGCgCGGUCGccuggacgaugaUGAuGAGGuUCCCc -3' miRNA: 3'- ugaCCACG-GCCAGC------------ACU-CUCUuAGGG- -5' |
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5594 | 3' | -55.1 | NC_001806.1 | + | 151321 | 0.66 | 0.921915 |
Target: 5'- uCUGGcGCCGGcUCGggcGGGGGGcuGUCCg -3' miRNA: 3'- uGACCaCGGCC-AGCa--CUCUCU--UAGGg -5' |
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5594 | 3' | -55.1 | NC_001806.1 | + | 19220 | 0.66 | 0.921915 |
Target: 5'- --cGG-GCCcgcuugGGUCGUGGGGGAAUgUCg -3' miRNA: 3'- ugaCCaCGG------CCAGCACUCUCUUAgGG- -5' |
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5594 | 3' | -55.1 | NC_001806.1 | + | 42750 | 0.66 | 0.921915 |
Target: 5'- -gUGGU-CCGGUUGU-AGcGGggUCCCg -3' miRNA: 3'- ugACCAcGGCCAGCAcUC-UCuuAGGG- -5' |
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5594 | 3' | -55.1 | NC_001806.1 | + | 66591 | 0.66 | 0.921915 |
Target: 5'- --cGGUGCCGuGaaUCGUGuuggugauccGGAGGcgCCCa -3' miRNA: 3'- ugaCCACGGC-C--AGCAC----------UCUCUuaGGG- -5' |
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5594 | 3' | -55.1 | NC_001806.1 | + | 117985 | 0.66 | 0.946635 |
Target: 5'- gGCUcgGGUGuuGGcCGUGAacgcGGGAUCCa -3' miRNA: 3'- -UGA--CCACggCCaGCACUc---UCUUAGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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