Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5604 | 5' | -61.4 | NC_001806.1 | + | 51118 | 0.74 | 0.23422 |
Target: 5'- uGCGGGCgcacgugucggggAGGCGCGcGaCCGGGCUGGGa -3' miRNA: 3'- cCGCCCG-------------UCCGCGUuC-GGCUCGACUU- -5' |
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5604 | 5' | -61.4 | NC_001806.1 | + | 21962 | 0.74 | 0.218504 |
Target: 5'- cGGcCGGGCAGccccgGCGGGUCGAGCUGGAc -3' miRNA: 3'- -CC-GCCCGUCcg---CGUUCGGCUCGACUU- -5' |
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5604 | 5' | -61.4 | NC_001806.1 | + | 117870 | 0.75 | 0.198314 |
Target: 5'- aGCGGGCGaugucGGCGgGGGCCGAGCccgUGAGc -3' miRNA: 3'- cCGCCCGU-----CCGCgUUCGGCUCG---ACUU- -5' |
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5604 | 5' | -61.4 | NC_001806.1 | + | 34395 | 0.77 | 0.151 |
Target: 5'- gGGUGGGC-GGCGguGGCCGGGCcGGg -3' miRNA: 3'- -CCGCCCGuCCGCguUCGGCUCGaCUu -5' |
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5604 | 5' | -61.4 | NC_001806.1 | + | 29675 | 0.81 | 0.073382 |
Target: 5'- aGGCGGGCGGGCgaggaaaggGCGGGCCGGGgaGGGg -3' miRNA: 3'- -CCGCCCGUCCG---------CGUUCGGCUCgaCUU- -5' |
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5604 | 5' | -61.4 | NC_001806.1 | + | 113762 | 0.97 | 0.00568 |
Target: 5'- gGGCGGGCAGG-GCAAGCCGAGCUGAAu -3' miRNA: 3'- -CCGCCCGUCCgCGUUCGGCUCGACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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