Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5639 | 3' | -51.8 | NC_001806.1 | + | 48124 | 0.69 | 0.916875 |
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Target: 5'- cCGCGCCUCUGGGUAcuuuauGGUGUugAUu -3' miRNA: 3'- cGUGCGGGGGUCCAUu-----UCACAugUAu -5' |
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| 5639 | 3' | -51.8 | NC_001806.1 | + | 80326 | 0.69 | 0.916875 |
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Target: 5'- uGCGCGCCCCCGGGg-------GCAUu -3' miRNA: 3'- -CGUGCGGGGGUCCauuucacaUGUAu -5' |
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| 5639 | 3' | -51.8 | NC_001806.1 | + | 116963 | 0.7 | 0.883793 |
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Target: 5'- cCugGCUCCCGuGGUucGGGUGUGCAc- -3' miRNA: 3'- cGugCGGGGGU-CCAu-UUCACAUGUau -5' |
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| 5639 | 3' | -51.8 | NC_001806.1 | + | 66998 | 0.7 | 0.876436 |
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Target: 5'- gGCGCGCCCCCAcccgcgucccgcGGUAGuuggccGUG-ACGUGg -3' miRNA: 3'- -CGUGCGGGGGU------------CCAUUu-----CACaUGUAU- -5' |
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| 5639 | 3' | -51.8 | NC_001806.1 | + | 22070 | 0.72 | 0.809658 |
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Target: 5'- gGCcccCGCCCCCGGGgcGGGUgcuGUACGg- -3' miRNA: 3'- -CGu--GCGGGGGUCCauUUCA---CAUGUau -5' |
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| 5639 | 3' | -51.8 | NC_001806.1 | + | 82217 | 0.72 | 0.800436 |
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Target: 5'- uCGCGCCCCCGGGggcguuGUGUggGCGa- -3' miRNA: 3'- cGUGCGGGGGUCCauuu--CACA--UGUau -5' |
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| 5639 | 3' | -51.8 | NC_001806.1 | + | 151738 | 0.73 | 0.752056 |
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Target: 5'- cGCGCGaCCCCCGGGg--GGUGUGu--- -3' miRNA: 3'- -CGUGC-GGGGGUCCauuUCACAUguau -5' |
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| 5639 | 3' | -51.8 | NC_001806.1 | + | 28 | 0.73 | 0.752056 |
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Target: 5'- cGCGCGaCCCCCGGGg--GGUGUGu--- -3' miRNA: 3'- -CGUGC-GGGGGUCCauuUCACAUguau -5' |
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| 5639 | 3' | -51.8 | NC_001806.1 | + | 34849 | 0.76 | 0.584295 |
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Target: 5'- cCACGCCCCCAGcGUcgGGUGUcACGg- -3' miRNA: 3'- cGUGCGGGGGUC-CAuuUCACA-UGUau -5' |
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| 5639 | 3' | -51.8 | NC_001806.1 | + | 89807 | 1 | 0.026134 |
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Target: 5'- gGCACGCCCCCAGG-AAAGUGUACAUAu -3' miRNA: 3'- -CGUGCGGGGGUCCaUUUCACAUGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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