miRNA: 3'- -UCCUCAGCGGCCUau-GCACCCGcaug -5'
miRNA: 3'- uCCUCAGCGGCcUAugCaCCCGCAUg -5'
miRNA: 3'- -UCCUCAGCGGCCUa-----------UGCACCCGCaug -5'
miRNA: 3'- -UCCUCAGCGGCCUAUGCACCCGCAUG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5647 | 3' | -57.2 | NC_001806.1 | + | 20995 | 0.73 | 0.490216 |
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Target: 5'- cGGGGGUCGUCGGGgucCGUGGGUc--- -3' miRNA: 3'- -UCCUCAGCGGCCUau-GCACCCGcaug -5' |
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| 5647 | 3' | -57.2 | NC_001806.1 | + | 28598 | 0.78 | 0.267908 |
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Target: 5'- cGGGGUCGCCGuGUugG-GGGCGUGg -3' miRNA: 3'- uCCUCAGCGGCcUAugCaCCCGCAUg -5' |
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| 5647 | 3' | -57.2 | NC_001806.1 | + | 33985 | 0.84 | 0.103287 |
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Target: 5'- cGGGGGUCGCCGGGgcaggggcggggGCGUGGGCGg-- -3' miRNA: 3'- -UCCUCAGCGGCCUa-----------UGCACCCGCaug -5' |
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| 5647 | 3' | -57.2 | NC_001806.1 | + | 84000 | 1.1 | 0.001972 |
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Target: 5'- gAGGAGUCGCCGGAUACGUGGGCGUACg -3' miRNA: 3'- -UCCUCAGCGGCCUAUGCACCCGCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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