miRNA: 3'- -UCCUCagCGg-----------CCUAUGCa-------CCCGCAUG- -5'
miRNA: 3'- -UCCuCAGCGgCCUA--UGCa-CCCGCAug -5'
miRNA: 3'- -UCCUCAGCGgCCua--UGCaCCCGCaug -5'
miRNA: 3'- -UCC---UCAGCGGCCuaUGCAcCCGcAUG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5647 | 3' | -57.2 | NC_001806.1 | + | 13142 | 0.66 | 0.880923 |
|
Target: 5'- gGGGAGagGCaggcugacggggGGAUGCGcagcgggaGGGCGUACu -3' miRNA: 3'- -UCCUCagCGg-----------CCUAUGCa-------CCCGCAUG- -5' |
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| 5647 | 3' | -57.2 | NC_001806.1 | + | 11320 | 0.68 | 0.757367 |
|
Target: 5'- uGGGuGUCGUgGGGUccACGggGGGCGUc- -3' miRNA: 3'- -UCCuCAGCGgCCUA--UGCa-CCCGCAug -5' |
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| 5647 | 3' | -57.2 | NC_001806.1 | + | 9192 | 0.7 | 0.628556 |
|
Target: 5'- cGGGGGUCGCgGGugugGCGaGGGCGc-- -3' miRNA: 3'- -UCCUCAGCGgCCua--UGCaCCCGCaug -5' |
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| 5647 | 3' | -57.2 | NC_001806.1 | + | 4750 | 0.71 | 0.60833 |
|
Target: 5'- gGGGcccGGUCGCCGGcgGCGUcGGC-UGCg -3' miRNA: 3'- -UCC---UCAGCGGCCuaUGCAcCCGcAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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