Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5658 | 5' | -55.9 | NC_001806.1 | + | 125855 | 0.69 | 0.789984 |
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Target: 5'- cCGGCCGcCGCGgACGCCGugGc--- -3' miRNA: 3'- cGCCGGCuGUGCaUGCGGCugUacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 47145 | 0.69 | 0.789984 |
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Target: 5'- uUGGUCGACGagcUGUuuGCCGACAUGa- -3' miRNA: 3'- cGCCGGCUGU---GCAugCGGCUGUACaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 81358 | 0.69 | 0.780808 |
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Target: 5'- gGCGGCCGGgGCGU-CGCCcGCGa--- -3' miRNA: 3'- -CGCCGGCUgUGCAuGCGGcUGUacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 20099 | 0.69 | 0.775238 |
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Target: 5'- cGUGGCCGGCGcCGUcugcgggcgucggucGCGCCGGgccuuUAUGUg -3' miRNA: 3'- -CGCCGGCUGU-GCA---------------UGCGGCU-----GUACAa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 43418 | 0.69 | 0.762065 |
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Target: 5'- uUGGCCcGCACGUaccACGCCuGCAUGg- -3' miRNA: 3'- cGCCGGcUGUGCA---UGCGGcUGUACaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 89603 | 0.69 | 0.752517 |
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Target: 5'- gGCGGCCGACaggagagugACGUA-GCCGguggGCGUGg- -3' miRNA: 3'- -CGCCGGCUG---------UGCAUgCGGC----UGUACaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 98541 | 0.7 | 0.742863 |
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Target: 5'- --cGCCGACGCcaACGCCGcCAUGUUu -3' miRNA: 3'- cgcCGGCUGUGcaUGCGGCuGUACAA- -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 76001 | 1.07 | 0.004074 |
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Target: 5'- cGCGGCCGACACGUACGCCGACAUGUUu -3' miRNA: 3'- -CGCCGGCUGUGCAUGCGGCUGUACAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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