Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5658 | 5' | -55.9 | NC_001806.1 | + | 118251 | 0.67 | 0.849776 |
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Target: 5'- -aGGCCGugGCGUuuguguGCGUcaccacccuCGGCAUGUg -3' miRNA: 3'- cgCCGGCugUGCA------UGCG---------GCUGUACAa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 125855 | 0.69 | 0.789984 |
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Target: 5'- cCGGCCGcCGCGgACGCCGugGc--- -3' miRNA: 3'- cGCCGGCuGUGCaUGCGGCugUacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 126130 | 0.66 | 0.912333 |
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Target: 5'- cGCGGaCGACgGCGgcgaggccgGCGCCGACAc--- -3' miRNA: 3'- -CGCCgGCUG-UGCa--------UGCGGCUGUacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 131878 | 0.66 | 0.918145 |
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Target: 5'- gGCGGCCGcccgcgcCGCGacgACGCCGGCc---- -3' miRNA: 3'- -CGCCGGCu------GUGCa--UGCGGCUGuacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 134832 | 0.71 | 0.69333 |
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Target: 5'- cGCGGCUGGCG-GU-CGCCGACcUGg- -3' miRNA: 3'- -CGCCGGCUGUgCAuGCGGCUGuACaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 136999 | 0.66 | 0.923718 |
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Target: 5'- gGCGGCCGGCGgGgaGCGCCagcuaGACGg--- -3' miRNA: 3'- -CGCCGGCUGUgCa-UGCGG-----CUGUacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 147756 | 0.67 | 0.872599 |
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Target: 5'- cGCGGgucCCGACGCGgcCGCgGACGc--- -3' miRNA: 3'- -CGCC---GGCUGUGCauGCGgCUGUacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 148116 | 0.71 | 0.683234 |
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Target: 5'- cGCGGCCGGCugGgGgGCUG-CGUGa- -3' miRNA: 3'- -CGCCGGCUGugCaUgCGGCuGUACaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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