Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5658 | 5' | -55.9 | NC_001806.1 | + | 9199 | 0.66 | 0.912333 |
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Target: 5'- cGCGGgUGugGCGaggGCGCgguCGACGUGg- -3' miRNA: 3'- -CGCCgGCugUGCa--UGCG---GCUGUACaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 6730 | 0.7 | 0.733113 |
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Target: 5'- -gGGCCcaGgACGUACGCCGACGUa-- -3' miRNA: 3'- cgCCGGc-UgUGCAUGCGGCUGUAcaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 4307 | 0.66 | 0.893492 |
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Target: 5'- aGCGGcCCGugGCGUcGCGgCCGGCc---- -3' miRNA: 3'- -CGCC-GGCugUGCA-UGC-GGCUGuacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 3802 | 0.76 | 0.40164 |
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Target: 5'- gGCGGCCGcCGCGUGCGCCaGGCc---- -3' miRNA: 3'- -CGCCGGCuGUGCAUGCGG-CUGuacaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 3465 | 0.72 | 0.632296 |
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Target: 5'- cGCGGCCGGCcuggGCGcgGCGCCcGGCGUcGUc -3' miRNA: 3'- -CGCCGGCUG----UGCa-UGCGG-CUGUA-CAa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 3004 | 0.68 | 0.825184 |
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Target: 5'- gGCGGCCGccaGCGCGU-CGgCGGCGUc-- -3' miRNA: 3'- -CGCCGGC---UGUGCAuGCgGCUGUAcaa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 2663 | 0.74 | 0.51169 |
|
Target: 5'- gGCGGCgGcCGCGgGCGCCGcCGUGUg -3' miRNA: 3'- -CGCCGgCuGUGCaUGCGGCuGUACAa -5' |
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| 5658 | 5' | -55.9 | NC_001806.1 | + | 2178 | 0.66 | 0.912333 |
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Target: 5'- aGC-GCCGACACGUcggggGCGCCGGu----- -3' miRNA: 3'- -CGcCGGCUGUGCA-----UGCGGCUguacaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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