Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5752 | 3' | -54.3 | NC_001806.1 | + | 66414 | 0.67 | 0.945066 |
Target: 5'- ---aCGuACaGGUGCGUgcgGUGGGGCGCc -3' miRNA: 3'- ggagGC-UG-CCACGCAa--UACCUCGUGc -5' |
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5752 | 3' | -54.3 | NC_001806.1 | + | 35497 | 0.67 | 0.945066 |
Target: 5'- uCCUCCGGagaGGgcgagGCGUc--GGAGCGgGg -3' miRNA: 3'- -GGAGGCUg--CCa----CGCAauaCCUCGUgC- -5' |
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5752 | 3' | -54.3 | NC_001806.1 | + | 148165 | 0.67 | 0.935751 |
Target: 5'- ---gCGGCGGcgccucUGCGUgggGGGGCGCGg -3' miRNA: 3'- ggagGCUGCC------ACGCAauaCCUCGUGC- -5' |
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5752 | 3' | -54.3 | NC_001806.1 | + | 90519 | 0.67 | 0.935751 |
Target: 5'- aCCUCaaacacgagaCGACGGgggagGCGcUGUGuGAGCGCc -3' miRNA: 3'- -GGAG----------GCUGCCa----CGCaAUAC-CUCGUGc -5' |
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5752 | 3' | -54.3 | NC_001806.1 | + | 57366 | 0.67 | 0.935751 |
Target: 5'- uCCUCCGGggacacggccCGGUGgcuguCGUUGcGGAGCAUc -3' miRNA: 3'- -GGAGGCU----------GCCAC-----GCAAUaCCUCGUGc -5' |
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5752 | 3' | -54.3 | NC_001806.1 | + | 13526 | 0.98 | 0.025615 |
Target: 5'- gCCUCCGACGGUGCGUUuaGGAGCACGc -3' miRNA: 3'- -GGAGGCUGCCACGCAAuaCCUCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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