Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5752 | 3' | -54.3 | NC_001806.1 | + | 50305 | 0.66 | 0.962312 |
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Target: 5'- cCCgacgCCGucCGGUGCGUUuugacgcgggaggccGccuuccUGGGGCGCGu -3' miRNA: 3'- -GGa---GGCu-GCCACGCAA---------------U------ACCUCGUGC- -5' |
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| 5752 | 3' | -54.3 | NC_001806.1 | + | 107714 | 0.66 | 0.96433 |
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Target: 5'- gCCUCacauCGGUGCGg---GGGGCAg- -3' miRNA: 3'- -GGAGgcu-GCCACGCaauaCCUCGUgc -5' |
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| 5752 | 3' | -54.3 | NC_001806.1 | + | 24221 | 0.66 | 0.96433 |
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Target: 5'- cCCgCCGACGGgcccGCGgUGUcgcGGcAGCACGc -3' miRNA: 3'- -GGaGGCUGCCa---CGCaAUA---CC-UCGUGC- -5' |
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| 5752 | 3' | -54.3 | NC_001806.1 | + | 138050 | 0.66 | 0.96433 |
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Target: 5'- aCCUCaUGAaGGUGC----UGGAGUACGg -3' miRNA: 3'- -GGAG-GCUgCCACGcaauACCUCGUGC- -5' |
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| 5752 | 3' | -54.3 | NC_001806.1 | + | 2824 | 0.66 | 0.967523 |
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Target: 5'- gCUCgGGCcccGGggGCGUggaggGGGGCGCGg -3' miRNA: 3'- gGAGgCUG---CCa-CGCAaua--CCUCGUGC- -5' |
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| 5752 | 3' | -54.3 | NC_001806.1 | + | 3338 | 0.66 | 0.967523 |
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Target: 5'- cCCUCCGGCc--GCGg---GGGGCugGc -3' miRNA: 3'- -GGAGGCUGccaCGCaauaCCUCGugC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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