Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5752 | 3' | -54.3 | NC_001806.1 | + | 30625 | 0.69 | 0.875056 |
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Target: 5'- cCCUCCGACucaGG-GCcggg-GGGGCGCGg -3' miRNA: 3'- -GGAGGCUG---CCaCGcaauaCCUCGUGC- -5' |
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| 5752 | 3' | -54.3 | NC_001806.1 | + | 129081 | 0.7 | 0.836421 |
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Target: 5'- gCC-CCGACGGccaggUGCGUgacUGUGGuccGCGCGc -3' miRNA: 3'- -GGaGGCUGCC-----ACGCA---AUACCu--CGUGC- -5' |
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| 5752 | 3' | -54.3 | NC_001806.1 | + | 132767 | 0.71 | 0.765445 |
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Target: 5'- gCCUCCugcccGCGGUGCGggAggcGGuGGCGCGg -3' miRNA: 3'- -GGAGGc----UGCCACGCaaUa--CC-UCGUGC- -5' |
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| 5752 | 3' | -54.3 | NC_001806.1 | + | 109403 | 0.72 | 0.716977 |
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Target: 5'- cCCgacCCGGCGaUGgGUcGUGGGGCGCGg -3' miRNA: 3'- -GGa--GGCUGCcACgCAaUACCUCGUGC- -5' |
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| 5752 | 3' | -54.3 | NC_001806.1 | + | 28714 | 0.73 | 0.69702 |
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Target: 5'- aCCcCCGAcucCGGUGCGUc-UGGAGgGCGg -3' miRNA: 3'- -GGaGGCU---GCCACGCAauACCUCgUGC- -5' |
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| 5752 | 3' | -54.3 | NC_001806.1 | + | 13526 | 0.98 | 0.025615 |
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Target: 5'- gCCUCCGACGGUGCGUUuaGGAGCACGc -3' miRNA: 3'- -GGAGGCUGCCACGCAAuaCCUCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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