Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5867 | 3' | -53.6 | NC_001806.1 | + | 1227 | 0.71 | 0.803389 |
Target: 5'- gUGGCgcaCCCGGACGUGgGG-CGAGAa- -3' miRNA: 3'- -ACCGa--GGGCUUGCGCgCUaGCUUUac -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 20112 | 0.66 | 0.970427 |
Target: 5'- cGGCgcgcguugccgagcaUCCCG-ACGCGCGGcUGgcGUGg -3' miRNA: 3'- aCCG---------------AGGGCuUGCGCGCUaGCuuUAC- -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 22187 | 0.75 | 0.572843 |
Target: 5'- cGGCUCCgGGGCGCGC--UCGGAGc- -3' miRNA: 3'- aCCGAGGgCUUGCGCGcuAGCUUUac -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 23711 | 0.67 | 0.951385 |
Target: 5'- cGGCggCCCGcguccAGgGCGCGGUCGuAGcgGg -3' miRNA: 3'- aCCGa-GGGC-----UUgCGCGCUAGC-UUuaC- -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 28861 | 0.67 | 0.937878 |
Target: 5'- cGGCuaaagUCCCGcGCGCGCuua-GAGAUGa -3' miRNA: 3'- aCCG-----AGGGCuUGCGCGcuagCUUUAC- -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 32083 | 0.75 | 0.593402 |
Target: 5'- uUGGC-CCCGAccaacGCGCGUGGUUGggGg- -3' miRNA: 3'- -ACCGaGGGCU-----UGCGCGCUAGCuuUac -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 37453 | 0.66 | 0.962769 |
Target: 5'- -uGUUCUCgGAGCGCGCG-UCGGAGg- -3' miRNA: 3'- acCGAGGG-CUUGCGCGCuAGCUUUac -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 38571 | 0.66 | 0.969237 |
Target: 5'- cGGgaCCUGGugGCGCacGUCGAGGc- -3' miRNA: 3'- aCCgaGGGCUugCGCGc-UAGCUUUac -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 51382 | 0.69 | 0.881391 |
Target: 5'- aGGCgccgCCCGAucgcgcacagcgcgACGUgcgcgaacagcguGCGAUCGggGUGc -3' miRNA: 3'- aCCGa---GGGCU--------------UGCG-------------CGCUAGCuuUAC- -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 51512 | 0.7 | 0.84629 |
Target: 5'- aGGUgcgCCCGGACaGCGCGAgcUCGGc--- -3' miRNA: 3'- aCCGa--GGGCUUG-CGCGCU--AGCUuuac -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 53667 | 0.74 | 0.614077 |
Target: 5'- gGGCgacCUgGGGCGCGCGGUCGGcaaGGUGg -3' miRNA: 3'- aCCGa--GGgCUUGCGCGCUAGCU---UUAC- -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 56871 | 0.7 | 0.854302 |
Target: 5'- gGaGCUCCCGGGgGCGgGggCGAGGa- -3' miRNA: 3'- aC-CGAGGGCUUgCGCgCuaGCUUUac -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 59583 | 0.76 | 0.512402 |
Target: 5'- cGGCUCCUGu-CGCGCGAggacaUCGAGAc- -3' miRNA: 3'- aCCGAGGGCuuGCGCGCU-----AGCUUUac -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 60478 | 0.69 | 0.891144 |
Target: 5'- cGGC-CCUGaAGCGCGCGGaCGGAu-- -3' miRNA: 3'- aCCGaGGGC-UUGCGCGCUaGCUUuac -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 61821 | 0.66 | 0.962769 |
Target: 5'- aGGC-CCCGAACcugaCGCGAcuuugCGAGcgGg -3' miRNA: 3'- aCCGaGGGCUUGc---GCGCUa----GCUUuaC- -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 65809 | 0.69 | 0.897831 |
Target: 5'- gGGC-CCUGGAgGUGCGGUUGAu--- -3' miRNA: 3'- aCCGaGGGCUUgCGCGCUAGCUuuac -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 73581 | 0.72 | 0.716943 |
Target: 5'- aGuGCUCCUGGGCGCGCccGUCGucGUGg -3' miRNA: 3'- aC-CGAGGGCUUGCGCGc-UAGCuuUAC- -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 76019 | 0.7 | 0.84629 |
Target: 5'- gGGCcgaCgCGcGGCGCGCGAUCGGAAg- -3' miRNA: 3'- aCCGa--GgGC-UUGCGCGCUAGCUUUac -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 76907 | 0.72 | 0.746773 |
Target: 5'- uUGGC-CCUGGACGCGCGggUGGc--- -3' miRNA: 3'- -ACCGaGGGCUUGCGCGCuaGCUuuac -5' |
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5867 | 3' | -53.6 | NC_001806.1 | + | 87661 | 0.66 | 0.969237 |
Target: 5'- aGGCgcugcaucUCCaCGAGCGCGUaGUUGAGAa- -3' miRNA: 3'- aCCG--------AGG-GCUUGCGCGcUAGCUUUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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