Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 5898 | 5' | -58 | NC_001806.1 | + | 119462 | 0.71 | 0.522722 |
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Target: 5'- cGCcg-CCACcCGGUCCgAGCGCGGaCCg -3' miRNA: 3'- -CGaaaGGUGcGCCAGG-UCGUGCC-GGa -5' |
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| 5898 | 5' | -58 | NC_001806.1 | + | 91894 | 0.71 | 0.522722 |
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Target: 5'- ----aCCAgGCGGUCCc-CACGGCCa -3' miRNA: 3'- cgaaaGGUgCGCCAGGucGUGCCGGa -5' |
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| 5898 | 5' | -58 | NC_001806.1 | + | 83820 | 0.72 | 0.493959 |
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Target: 5'- gGCggggUCgGCGCGGgaUCCGGCACGcaGCCg -3' miRNA: 3'- -CGaa--AGgUGCGCC--AGGUCGUGC--CGGa -5' |
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| 5898 | 5' | -58 | NC_001806.1 | + | 100120 | 0.72 | 0.46594 |
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Target: 5'- cGCccgCCugGCGGUCCgcGGCuAUGGCCc -3' miRNA: 3'- -CGaaaGGugCGCCAGG--UCG-UGCCGGa -5' |
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| 5898 | 5' | -58 | NC_001806.1 | + | 92899 | 0.75 | 0.347512 |
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Target: 5'- gGCUgcggCCGCGUGGUCCGcGCAgGGCg- -3' miRNA: 3'- -CGAaa--GGUGCGCCAGGU-CGUgCCGga -5' |
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| 5898 | 5' | -58 | NC_001806.1 | + | 122443 | 0.76 | 0.310756 |
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Target: 5'- ---cUCCGCGUGGUCCccgaggAGCACGGCg- -3' miRNA: 3'- cgaaAGGUGCGCCAGG------UCGUGCCGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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