Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5926 | 5' | -55.9 | NC_001806.1 | + | 135062 | 0.67 | 0.881141 |
Target: 5'- aCCGGcccCGCGggGA--CGCGCCGGGc -3' miRNA: 3'- -GGCCca-GUGCuaCUuaGCGCGGUCCa -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 146874 | 0.66 | 0.924416 |
Target: 5'- gCCGGGUCGCcAUGuuUCcCGUCuGGUc -3' miRNA: 3'- -GGCCCAGUGcUACuuAGcGCGGuCCA- -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 150981 | 0.7 | 0.74614 |
Target: 5'- gCGGGccCACGAgccgcggCGCGCCAGGc -3' miRNA: 3'- gGCCCa-GUGCUacuua--GCGCGGUCCa -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 151075 | 0.67 | 0.881141 |
Target: 5'- -gGGGUCGCGG-GggUCGCGg-GGGUc -3' miRNA: 3'- ggCCCAGUGCUaCuuAGCGCggUCCA- -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 151102 | 0.68 | 0.843689 |
Target: 5'- -gGGGUCGCGG-GggUCGCGggGGGc -3' miRNA: 3'- ggCCCAGUGCUaCuuAGCGCggUCCa -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 151144 | 0.66 | 0.934696 |
Target: 5'- cCCGcGcGUCGCaGgcGcAggCGCGCCAGGUg -3' miRNA: 3'- -GGC-C-CAGUG-CuaC-UuaGCGCGGUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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