Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5926 | 5' | -55.9 | NC_001806.1 | + | 34415 | 0.66 | 0.924416 |
Target: 5'- gCCGGGcCGgGccGGGcCGgGCCGGGUg -3' miRNA: 3'- -GGCCCaGUgCuaCUUaGCgCGGUCCA- -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 2569 | 0.66 | 0.924416 |
Target: 5'- gCGGGUCAaacAUGAGggcCGguCGCCAGGg -3' miRNA: 3'- gGCCCAGUgc-UACUUa--GC--GCGGUCCa -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 25683 | 0.66 | 0.9132 |
Target: 5'- -aGGa--GCGGUGuGUUGCGCCGGGg -3' miRNA: 3'- ggCCcagUGCUACuUAGCGCGGUCCa -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 50645 | 0.67 | 0.901056 |
Target: 5'- cCCGGGcgUGCGggGAcgCgcaccgGCGCCGGGa -3' miRNA: 3'- -GGCCCa-GUGCuaCUuaG------CGCGGUCCa -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 22635 | 0.67 | 0.901056 |
Target: 5'- aCCGGGcgagCGCGcgGug-CcCGCCGGGUa -3' miRNA: 3'- -GGCCCa---GUGCuaCuuaGcGCGGUCCA- -5' |
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5926 | 5' | -55.9 | NC_001806.1 | + | 113168 | 0.76 | 0.43338 |
Target: 5'- gCGGGUC-CGAUGggUCGCggaagGCCAGa- -3' miRNA: 3'- gGCCCAGuGCUACuuAGCG-----CGGUCca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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