Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
5934 | 5' | -57.9 | NC_001806.1 | + | 34384 | 0.66 | 0.825149 |
Target: 5'- --cCGAGGUGggUGGGUGGGcGGcGGu -3' miRNA: 3'- cuaGCUCCGCuaGCUCACCCaCC-CCu -5' |
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5934 | 5' | -57.9 | NC_001806.1 | + | 20711 | 0.66 | 0.833399 |
Target: 5'- -uUCGGGGUGGgcccgcCGGGgGGGcGGGGGg -3' miRNA: 3'- cuAGCUCCGCUa-----GCUCaCCCaCCCCU- -5' |
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5934 | 5' | -57.9 | NC_001806.1 | + | 111222 | 0.66 | 0.849363 |
Target: 5'- --cCGGGGCc-UCGAuGUGGGUGGcGGc -3' miRNA: 3'- cuaGCUCCGcuAGCU-CACCCACC-CCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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