Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5979 | 5' | -54.8 | NC_001806.1 | + | 92930 | 0.67 | 0.910358 |
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Target: 5'- gCGGgccugGAGGc-CGGGgCCCGCGCGc -3' miRNA: 3'- -GCCauua-CUCUauGCUCgGGGCGCGC- -5' |
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| 5979 | 5' | -54.8 | NC_001806.1 | + | 96738 | 0.7 | 0.768149 |
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Target: 5'- aCGGUGGUGcAGuu-CGAGCagCCGCGCc -3' miRNA: 3'- -GCCAUUAC-UCuauGCUCGg-GGCGCGc -5' |
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| 5979 | 5' | -54.8 | NC_001806.1 | + | 99931 | 0.69 | 0.822226 |
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Target: 5'- uGGUcccggGAGAaugGCGGGCCgCGCGCc -3' miRNA: 3'- gCCAuua--CUCUa--UGCUCGGgGCGCGc -5' |
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| 5979 | 5' | -54.8 | NC_001806.1 | + | 121566 | 0.72 | 0.709722 |
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Target: 5'- cCGGg---GGGcUGCGAGUCCUGCGCc -3' miRNA: 3'- -GCCauuaCUCuAUGCUCGGGGCGCGc -5' |
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| 5979 | 5' | -54.8 | NC_001806.1 | + | 136960 | 0.69 | 0.854938 |
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Target: 5'- aCGcGUAGUGGGGgcccGCGAGCgUgGUGCGg -3' miRNA: 3'- -GC-CAUUACUCUa---UGCUCGgGgCGCGC- -5' |
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| 5979 | 5' | -54.8 | NC_001806.1 | + | 139782 | 0.71 | 0.758661 |
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Target: 5'- aGGUGGugcauguuuuUGAG-UACG-GCaCCCGCGCGa -3' miRNA: 3'- gCCAUU----------ACUCuAUGCuCG-GGGCGCGC- -5' |
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| 5979 | 5' | -54.8 | NC_001806.1 | + | 141467 | 0.68 | 0.865647 |
|
Target: 5'- aCGGUcccaaccgauucuagAGUGGGAcGCcgccGCCCCGCGCu -3' miRNA: 3'- -GCCA---------------UUACUCUaUGcu--CGGGGCGCGc -5' |
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| 5979 | 5' | -54.8 | NC_001806.1 | + | 147344 | 0.66 | 0.941706 |
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Target: 5'- uCGGaGggGGGGUGCGucgccgcccucuuGGCCCCugccgGCGCGa -3' miRNA: 3'- -GCCaUuaCUCUAUGC-------------UCGGGG-----CGCGC- -5' |
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| 5979 | 5' | -54.8 | NC_001806.1 | + | 151044 | 0.68 | 0.870111 |
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Target: 5'- cCGGacGUGGG--GCGAGaagcgcaCCCGCGCGg -3' miRNA: 3'- -GCCauUACUCuaUGCUCg------GGGCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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