Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5988 | 5' | -51.8 | NC_001806.1 | + | 97556 | 0.66 | 0.990869 |
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Target: 5'- aCCaCCAaccUGaCCGAGUAcccgcucucgcgcgUGGAcCUGGGGGa -3' miRNA: 3'- -GG-GGU---AC-GGUUCAUa-------------ACCU-GACCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 98198 | 0.67 | 0.980056 |
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Target: 5'- gCCCGUugGUCGAGg---GGcaGCUGGGGGa -3' miRNA: 3'- gGGGUA--CGGUUCauaaCC--UGACCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 101473 | 0.66 | 0.991577 |
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Target: 5'- aCgCCAaccGCCGGGccg-GGACUGGAGc -3' miRNA: 3'- -GgGGUa--CGGUUCauaaCCUGACCUCc -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 143235 | 0.71 | 0.897814 |
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Target: 5'- aCCCGUGCUgguGGUGcgGG-UUGGGGGg -3' miRNA: 3'- gGGGUACGGu--UCAUaaCCuGACCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 144018 | 0.69 | 0.951733 |
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Target: 5'- aCCCCAacggaaagcgGCgGGGUGUUGGg--GGAGGc -3' miRNA: 3'- -GGGGUa---------CGgUUCAUAACCugaCCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 147329 | 0.66 | 0.985925 |
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Target: 5'- cCCCCcgGCCcuGAGUc--GGAggGGGGGu -3' miRNA: 3'- -GGGGuaCGG--UUCAuaaCCUgaCCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 147374 | 0.76 | 0.632164 |
|
Target: 5'- gCCCC-UGCCGgcgcgaggggGGgacgcgUGGACUGGGGGg -3' miRNA: 3'- -GGGGuACGGU----------UCaua---ACCUGACCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 147680 | 0.7 | 0.927876 |
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Target: 5'- gCCCCGgggGCCGGGgcgcggGGGCgggccccGGAGGc -3' miRNA: 3'- -GGGGUa--CGGUUCauaa--CCUGa------CCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 151266 | 0.68 | 0.966495 |
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Target: 5'- gCCCAcGCCGggcGGUGggggccgGGGCcGGGGGg -3' miRNA: 3'- gGGGUaCGGU---UCAUaa-----CCUGaCCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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