Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5988 | 5' | -51.8 | NC_001806.1 | + | 147329 | 0.66 | 0.985925 |
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Target: 5'- cCCCCcgGCCcuGAGUc--GGAggGGGGGu -3' miRNA: 3'- -GGGGuaCGG--UUCAuaaCCUgaCCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 13121 | 0.66 | 0.985925 |
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Target: 5'- aCCCCcUGCU-GGUA--GGcCUGGGGGg -3' miRNA: 3'- -GGGGuACGGuUCAUaaCCuGACCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 81823 | 0.66 | 0.984138 |
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Target: 5'- aCCCCGcaggGCCu-GUGccacGGGCgGGAGGg -3' miRNA: 3'- -GGGGUa---CGGuuCAUaa--CCUGaCCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 50875 | 0.66 | 0.984138 |
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Target: 5'- aCCCccGCCAAGUucUGGGC-GGAc- -3' miRNA: 3'- gGGGuaCGGUUCAuaACCUGaCCUcc -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 3338 | 0.66 | 0.984138 |
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Target: 5'- cCCUCcgGCCGcGgg--GGGCUGGcGGg -3' miRNA: 3'- -GGGGuaCGGUuCauaaCCUGACCuCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 14123 | 0.67 | 0.982185 |
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Target: 5'- uCCCCcUGCCGGaca-UGGuuUGGGGGg -3' miRNA: 3'- -GGGGuACGGUUcauaACCugACCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 98198 | 0.67 | 0.980056 |
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Target: 5'- gCCCGUugGUCGAGg---GGcaGCUGGGGGa -3' miRNA: 3'- gGGGUA--CGGUUCauaaCC--UGACCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 26690 | 0.67 | 0.977743 |
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Target: 5'- cCCCCAccGCCcGGcg-UGGGCccGGGGGg -3' miRNA: 3'- -GGGGUa-CGGuUCauaACCUGa-CCUCC- -5' |
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| 5988 | 5' | -51.8 | NC_001806.1 | + | 147374 | 0.76 | 0.632164 |
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Target: 5'- gCCCC-UGCCGgcgcgaggggGGgacgcgUGGACUGGGGGg -3' miRNA: 3'- -GGGGuACGGU----------UCaua---ACCUGACCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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