Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 5994 | 3' | -57.1 | NC_001806.1 | + | 23129 | 0.67 | 0.817734 |
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Target: 5'- gCCGCGggaCCCGCgCCUGccGAGCUCc- -3' miRNA: 3'- gGGCGUauaGGGUG-GGAC--CUCGAGuu -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 27638 | 0.67 | 0.808998 |
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Target: 5'- aCCCGCAUuggUC-CCCUGGGGUUCc- -3' miRNA: 3'- -GGGCGUAuagGGuGGGACCUCGAGuu -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 28118 | 0.67 | 0.800102 |
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Target: 5'- aCCCcCAUGgagcCCCGCCCcGGAGCg--- -3' miRNA: 3'- -GGGcGUAUa---GGGUGGGaCCUCGaguu -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 119299 | 0.67 | 0.781861 |
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Target: 5'- gCCCGCAga-CCCGCCCaucgucGGGGCgucCGAc -3' miRNA: 3'- -GGGCGUauaGGGUGGGa-----CCUCGa--GUU- -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 137230 | 0.67 | 0.772533 |
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Target: 5'- cCCCGCAgg-CCUGCCCcucagGGAGUUUc- -3' miRNA: 3'- -GGGCGUauaGGGUGGGa----CCUCGAGuu -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 135618 | 0.67 | 0.772533 |
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Target: 5'- cCCCGC-UGUUgCGCgCC-GGAGCUCGc -3' miRNA: 3'- -GGGCGuAUAGgGUG-GGaCCUCGAGUu -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 128696 | 0.68 | 0.76308 |
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Target: 5'- cCCCGCGca-CCUcgGCCgUGGAGUUCAc -3' miRNA: 3'- -GGGCGUauaGGG--UGGgACCUCGAGUu -5' |
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| 5994 | 3' | -57.1 | NC_001806.1 | + | 94229 | 0.72 | 0.492503 |
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Target: 5'- cCCCGC-UGUCggUCACCCUGGAGUUg-- -3' miRNA: 3'- -GGGCGuAUAG--GGUGGGACCUCGAguu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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