Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
600 | 3' | -49.5 | AC_000017.1 | + | 11451 | 0.66 | 0.916362 |
Target: 5'- aCUUUGAgCCCGacGCGCGGAccgGGAUuaguCCc -3' miRNA: 3'- -GAAACU-GGGCcuUGCGCCU---UUUGu---GG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 25738 | 0.66 | 0.909612 |
Target: 5'- --gUGACCCcaaAGCgGCGGAGGGCAUUc -3' miRNA: 3'- gaaACUGGGcc-UUG-CGCCUUUUGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 18988 | 0.66 | 0.909612 |
Target: 5'- ---cGuCUCGGuGGCGCGGGcgaacuGCACCa -3' miRNA: 3'- gaaaCuGGGCC-UUGCGCCUuu----UGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 21276 | 0.66 | 0.909612 |
Target: 5'- --aUGAgCCGucaGGugGUGGAcGAUACCa -3' miRNA: 3'- gaaACUgGGC---CUugCGCCUuUUGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 25532 | 0.66 | 0.902554 |
Target: 5'- ---cGGCCaCGGAGCGCucguuGAAgGCCg -3' miRNA: 3'- gaaaCUGG-GCCUUGCGccu--UUUgUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 12128 | 0.66 | 0.902554 |
Target: 5'- aCUUUGACgCGG-GCGCuGAccuGCGCUg -3' miRNA: 3'- -GAAACUGgGCCuUGCGcCUuu-UGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 6632 | 0.66 | 0.901831 |
Target: 5'- -gUUG-CCCGGGaucuuuaccguggACGCGGAccccccggacgAGACGCa -3' miRNA: 3'- gaAACuGGGCCU-------------UGCGCCU-----------UUUGUGg -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 26598 | 0.66 | 0.895191 |
Target: 5'- ----cGCUCGuGGCGCGGGcacaaGAACGCCa -3' miRNA: 3'- gaaacUGGGCcUUGCGCCU-----UUUGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 9724 | 0.66 | 0.895191 |
Target: 5'- ---cGAUCCGGucGAUGCGGAcu-CGCUc -3' miRNA: 3'- gaaaCUGGGCC--UUGCGCCUuuuGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 5491 | 0.67 | 0.890629 |
Target: 5'- --aUGGCCCuuGGCGCGcagcuugcccuuggaGGAGGCGCCg -3' miRNA: 3'- gaaACUGGGccUUGCGC---------------CUUUUGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 23443 | 0.67 | 0.887529 |
Target: 5'- ---aGACCUGGcugAACGaGGA--GCACCg -3' miRNA: 3'- gaaaCUGGGCC---UUGCgCCUuuUGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 28000 | 0.67 | 0.887529 |
Target: 5'- ------aCCGGAGcCGCGGGcaAAGCACUu -3' miRNA: 3'- gaaacugGGCCUU-GCGCCU--UUUGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 31267 | 0.67 | 0.887529 |
Target: 5'- --gUGGuuCGGAaagGCGCaGaGAAAGCACCc -3' miRNA: 3'- gaaACUggGCCU---UGCG-C-CUUUUGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 9266 | 0.67 | 0.887529 |
Target: 5'- ---aGGCCaUGGAGCGCcuugaggccuuGGggGAUAUCa -3' miRNA: 3'- gaaaCUGG-GCCUUGCG-----------CCuuUUGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 3755 | 0.67 | 0.877129 |
Target: 5'- -cUUGACCUacgagaccgugucuGGAACGCcguuGGAGACugCa -3' miRNA: 3'- gaAACUGGG--------------CCUUGCGc---CUUUUGugG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 5336 | 0.67 | 0.871329 |
Target: 5'- --aUGACCCuGGccCGUcuGGAcGAGCACCg -3' miRNA: 3'- gaaACUGGG-CCuuGCG--CCU-UUUGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 5404 | 0.67 | 0.869647 |
Target: 5'- ----aGCCCGGAGCGCaccccuuCACCg -3' miRNA: 3'- gaaacUGGGCCUUGCGccuuuu-GUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 12193 | 0.67 | 0.85401 |
Target: 5'- ---gGACCUGGGcugGCGGu-GGCACCc -3' miRNA: 3'- gaaaCUGGGCCUug-CGCCuuUUGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 6043 | 0.67 | 0.85401 |
Target: 5'- ----cACCCGGucACGUGGcauACACCu -3' miRNA: 3'- gaaacUGGGCCu-UGCGCCuuuUGUGG- -5' |
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600 | 3' | -49.5 | AC_000017.1 | + | 362 | 0.68 | 0.844953 |
Target: 5'- aCUUUGACCguuuACGUGGAGAcuCGCCc -3' miRNA: 3'- -GAAACUGGgccuUGCGCCUUUu-GUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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