Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6001 | 5' | -58.1 | NC_001806.1 | + | 15055 | 0.66 | 0.825149 |
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Target: 5'- aUGGUGGGGGGuGUC-UUCGGGCGAc- -3' miRNA: 3'- -ACCAUCUCCUcCGGcAGGCCUGCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 34405 | 0.66 | 0.825149 |
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Target: 5'- cGGUGGccGGGccGGGCCGggCCGGGcCGGg- -3' miRNA: 3'- aCCAUC--UCC--UCCGGCa-GGCCU-GCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 11818 | 0.66 | 0.825149 |
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Target: 5'- gGGUGGAGGuGGUgGUCgagggGGugGAg- -3' miRNA: 3'- aCCAUCUCCuCCGgCAGg----CCugCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 76910 | 0.66 | 0.808147 |
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Target: 5'- aGGUcucucGAGGAGGCCGaggcggagUgGGACGAg- -3' miRNA: 3'- aCCAu----CUCCUCCGGCa-------GgCCUGCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 22142 | 0.68 | 0.744222 |
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Target: 5'- aGGcGGAGGAGGCgCGacgCCGGuuCGAg- -3' miRNA: 3'- aCCaUCUCCUCCG-GCa--GGCCu-GCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 75212 | 0.68 | 0.734641 |
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Target: 5'- cGGUGGAuGcGGCCGUgCGGGCGc-- -3' miRNA: 3'- aCCAUCUcCuCCGGCAgGCCUGCuag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 48598 | 0.68 | 0.734641 |
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Target: 5'- cGGUGGguAGGGGGCgCGaCCGGACc--- -3' miRNA: 3'- aCCAUC--UCCUCCG-GCaGGCCUGcuag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 34309 | 0.68 | 0.724975 |
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Target: 5'- cGGcGGGGGGcGGCgCG-CCGGACGGg- -3' miRNA: 3'- aCCaUCUCCU-CCG-GCaGGCCUGCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 151278 | 0.68 | 0.724975 |
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Target: 5'- cGGUGGGGGccgGGGCCGgg-GGGCGGc- -3' miRNA: 3'- aCCAUCUCC---UCCGGCaggCCUGCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 21380 | 1.08 | 0.00209 |
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Target: 5'- aUGGUAGAGGAGGCCGUCCGGACGAUCc -3' miRNA: 3'- -ACCAUCUCCUCCGGCAGGCCUGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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