Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6001 | 5' | -58.1 | NC_001806.1 | + | 74057 | 0.74 | 0.381904 |
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Target: 5'- cGGcUGGAGGAGGugcggacccaggcCCGU-CGGGCGAUCg -3' miRNA: 3'- aCC-AUCUCCUCC-------------GGCAgGCCUGCUAG- -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 75212 | 0.68 | 0.734641 |
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Target: 5'- cGGUGGAuGcGGCCGUgCGGGCGc-- -3' miRNA: 3'- aCCAUCUcCuCCGGCAgGCCUGCuag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 76910 | 0.66 | 0.808147 |
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Target: 5'- aGGUcucucGAGGAGGCCGaggcggagUgGGACGAg- -3' miRNA: 3'- aCCAu----CUCCUCCGGCa-------GgCCUGCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 99606 | 0.74 | 0.407655 |
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Target: 5'- cGGUAGAGGGGcGCgCGUCUgGGugGAa- -3' miRNA: 3'- aCCAUCUCCUC-CG-GCAGG-CCugCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 99737 | 0.68 | 0.705418 |
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Target: 5'- gUGGUGGGGGGcgauacacGGCC-UCCGGGgGAc- -3' miRNA: 3'- -ACCAUCUCCU--------CCGGcAGGCCUgCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 100616 | 0.66 | 0.849363 |
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Target: 5'- --cUGGAGGuAGGUgUGUCCGG-CGAUCc -3' miRNA: 3'- accAUCUCC-UCCG-GCAGGCCuGCUAG- -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 146069 | 0.7 | 0.585363 |
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Target: 5'- cGGccAGAGGccAGGUCaGUCCGGGCGggCa -3' miRNA: 3'- aCCa-UCUCC--UCCGG-CAGGCCUGCuaG- -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 147270 | 0.66 | 0.841473 |
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Target: 5'- aGGaAGAggcGGAGGCCG-CCgaGGACG-UCa -3' miRNA: 3'- aCCaUCU---CCUCCGGCaGG--CCUGCuAG- -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 151278 | 0.68 | 0.724975 |
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Target: 5'- cGGUGGGGGccgGGGCCGgg-GGGCGGc- -3' miRNA: 3'- aCCAUCUCC---UCCGGCaggCCUGCUag -5' |
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| 6001 | 5' | -58.1 | NC_001806.1 | + | 151334 | 0.71 | 0.555598 |
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Target: 5'- cGGgcG-GGGGGCUGUCCGGcCaGUCg -3' miRNA: 3'- aCCauCuCCUCCGGCAGGCCuGcUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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