Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6050 | 5' | -53.1 | NC_001815.1 | + | 1116 | 0.66 | 0.310383 |
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Target: 5'- cGGAG-GAGcCGAGGGCUguUGGAGgGGCg -3' miRNA: 3'- -CCUCuCUU-GUUCCUGG--GCUUCaCCGa -5' |
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| 6050 | 5' | -53.1 | NC_001815.1 | + | 2225 | 0.68 | 0.217039 |
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Target: 5'- --cGGGGGCAAGGGCCUGggGcaggacGGUg -3' miRNA: 3'- ccuCUCUUGUUCCUGGGCuuCa-----CCGa -5' |
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| 6050 | 5' | -53.1 | NC_001815.1 | + | 2490 | 0.69 | 0.191725 |
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Target: 5'- cGGGGAGGAguuauuccauCGGGGAgCCGggG-GGUa -3' miRNA: 3'- -CCUCUCUU----------GUUCCUgGGCuuCaCCGa -5' |
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| 6050 | 5' | -53.1 | NC_001815.1 | + | 4333 | 0.69 | 0.174475 |
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Target: 5'- uGAGGaAACAAGGGCCCuGGAGUuGCa -3' miRNA: 3'- cCUCUcUUGUUCCUGGG-CUUCAcCGa -5' |
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| 6050 | 5' | -53.1 | NC_001815.1 | + | 7026 | 0.7 | 0.169039 |
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Target: 5'- cGGAGAGGAUGuacGGGAUggucgCCGGAG-GGCg -3' miRNA: 3'- -CCUCUCUUGU---UCCUG-----GGCUUCaCCGa -5' |
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| 6050 | 5' | -53.1 | NC_001815.1 | + | 2349 | 0.75 | 0.069753 |
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Target: 5'- gGGAGGGuAACAGGGGCCCuccgGAAG-GGUa -3' miRNA: 3'- -CCUCUC-UUGUUCCUGGG----CUUCaCCGa -5' |
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| 6050 | 5' | -53.1 | NC_001815.1 | + | 707 | 1.11 | 8.4e-05 |
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Target: 5'- cGGAGAGAACAAGGACCCGAAGUGGCUg -3' miRNA: 3'- -CCUCUCUUGUUCCUGGGCUUCACCGA- -5' |
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| 6050 | 5' | -53.1 | NC_001815.1 | + | 8842 | 1.11 | 8.4e-05 |
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Target: 5'- cGGAGAGAACAAGGACCCGAAGUGGCUg -3' miRNA: 3'- -CCUCUCUUGUUCCUGGGCUUCACCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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