Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
615 | 5' | -45.8 | AC_000017.1 | + | 14761 | 0.65 | 0.990751 |
Target: 5'- aGCGACaUCGggguaaaguuugacACCCgcaacuucagacUGGGGuuUGACCCa -3' miRNA: 3'- -CGCUG-AGU--------------UGGGa-----------ACCUUuuAUUGGG- -5' |
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615 | 5' | -45.8 | AC_000017.1 | + | 27588 | 0.66 | 0.99002 |
Target: 5'- gGCGAC-CGcACCCUgugacGAAAGccGCCCg -3' miRNA: 3'- -CGCUGaGU-UGGGAac---CUUUUauUGGG- -5' |
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615 | 5' | -45.8 | AC_000017.1 | + | 5962 | 0.66 | 0.99002 |
Target: 5'- aGCGAgUgGACCCccuagUGGAcAAcGACCg -3' miRNA: 3'- -CGCUgAgUUGGGa----ACCUuUUaUUGGg -5' |
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615 | 5' | -45.8 | AC_000017.1 | + | 7138 | 0.66 | 0.984637 |
Target: 5'- cGCGGuCUUuccaguACUCUUGGAucggaAACCCg -3' miRNA: 3'- -CGCU-GAGu-----UGGGAACCUuuua-UUGGG- -5' |
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615 | 5' | -45.8 | AC_000017.1 | + | 3215 | 0.66 | 0.984637 |
Target: 5'- cUGGC-CAGgCCUUGcGAGAGUGGCUg -3' miRNA: 3'- cGCUGaGUUgGGAAC-CUUUUAUUGGg -5' |
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615 | 5' | -45.8 | AC_000017.1 | + | 33126 | 0.66 | 0.984637 |
Target: 5'- uUGGCUCGACaggaaaCCgugUGGAAuAUAACaCCu -3' miRNA: 3'- cGCUGAGUUG------GGa--ACCUUuUAUUG-GG- -5' |
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615 | 5' | -45.8 | AC_000017.1 | + | 11328 | 0.66 | 0.983997 |
Target: 5'- cGCGGCagAACCUguuucgugaccgcgaGGGAGAggAGCCCg -3' miRNA: 3'- -CGCUGagUUGGGaa-------------CCUUUUa-UUGGG- -5' |
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615 | 5' | -45.8 | AC_000017.1 | + | 1983 | 0.7 | 0.90126 |
Target: 5'- aUGAC-CuuCUCUUGGAAAagcgccugGUGACCCa -3' miRNA: 3'- cGCUGaGuuGGGAACCUUU--------UAUUGGG- -5' |
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615 | 5' | -45.8 | AC_000017.1 | + | 5368 | 0.71 | 0.884827 |
Target: 5'- cGCuGACgagGACCCUgcgcccgUGGAAAgacAUGACCCu -3' miRNA: 3'- -CG-CUGag-UUGGGA-------ACCUUU---UAUUGGG- -5' |
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615 | 5' | -45.8 | AC_000017.1 | + | 5485 | 0.71 | 0.86965 |
Target: 5'- cGCGGCaUGGCCCUUGGcgcgcagcuuGCCCu -3' miRNA: 3'- -CGCUGaGUUGGGAACCuuuuau----UGGG- -5' |
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615 | 5' | -45.8 | AC_000017.1 | + | 35373 | 0.71 | 0.859901 |
Target: 5'- aGCGACcgaaaUAGCCCg-GGGGAAUAcauACCCg -3' miRNA: 3'- -CGCUGa----GUUGGGaaCCUUUUAU---UGGG- -5' |
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615 | 5' | -45.8 | AC_000017.1 | + | 11348 | 0.71 | 0.859901 |
Target: 5'- cGCGugUCAcgcuucagcugcACCCUUGGGu-GUcGCUCa -3' miRNA: 3'- -CGCugAGU------------UGGGAACCUuuUAuUGGG- -5' |
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615 | 5' | -45.8 | AC_000017.1 | + | 10957 | 1.17 | 0.002084 |
Target: 5'- cGCGACUCAACCCUUGGAAAAUAACCCu -3' miRNA: 3'- -CGCUGAGUUGGGAACCUUUUAUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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