Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6197 | 5' | -51.9 | NC_001844.1 | + | 121869 | 0.68 | 0.957808 |
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Target: 5'- -aGUGGGCgCAAAAaaGGAGGcGGGUGCu -3' miRNA: 3'- cgUACCUG-GUUUUcgCCUCU-UCCGCG- -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 126101 | 0.67 | 0.971293 |
|
Target: 5'- gGCAUGgGACCGGuuuuGCGGGGuucugcauagcaGAGGuCGUc -3' miRNA: 3'- -CGUAC-CUGGUUuu--CGCCUC------------UUCC-GCG- -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 138759 | 0.7 | 0.907053 |
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Target: 5'- ---cGGugUgcguGUGGGGggGGCGCa -3' miRNA: 3'- cguaCCugGuuuuCGCCUCuuCCGCG- -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 139626 | 0.67 | 0.974113 |
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Target: 5'- cGCGUGcGuuauuucCCAgGAAGCGGAaauGGCGCa -3' miRNA: 3'- -CGUAC-Cu------GGU-UUUCGCCUcuuCCGCG- -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 140863 | 0.77 | 0.560219 |
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Target: 5'- cGCG-GGGCuCGGGGGcCGGGGAAGGCGg -3' miRNA: 3'- -CGUaCCUG-GUUUUC-GCCUCUUCCGCg -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 143035 | 0.73 | 0.786608 |
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Target: 5'- -aGUGGGCCAcAAGGCGGGGAugcgggAGGUcugGCu -3' miRNA: 3'- cgUACCUGGU-UUUCGCCUCU------UCCG---CG- -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 144224 | 0.67 | 0.965005 |
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Target: 5'- ---aGGACCGcgcGGCuGAGguGGCGCu -3' miRNA: 3'- cguaCCUGGUuu-UCGcCUCuuCCGCG- -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 145530 | 0.7 | 0.893196 |
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Target: 5'- -gGUGuGACCGGAAGCGGAagugacgcaagcgGAAGGgGa -3' miRNA: 3'- cgUAC-CUGGUUUUCGCCU-------------CUUCCgCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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