Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6197 | 5' | -51.9 | NC_001844.1 | + | 126101 | 0.67 | 0.971293 |
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Target: 5'- gGCAUGgGACCGGuuuuGCGGGGuucugcauagcaGAGGuCGUc -3' miRNA: 3'- -CGUAC-CUGGUUuu--CGCCUC------------UUCC-GCG- -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 1149 | 0.67 | 0.968259 |
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Target: 5'- -gGUGGGCaCAcAGAGaUGGAuaccgaGGAGGCGCg -3' miRNA: 3'- cgUACCUG-GU-UUUC-GCCU------CUUCCGCG- -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 61662 | 0.67 | 0.968259 |
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Target: 5'- -uGUGGAuCCAGuAGGCGGcGGGcAGGUGUa -3' miRNA: 3'- cgUACCU-GGUU-UUCGCC-UCU-UCCGCG- -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 144224 | 0.67 | 0.965005 |
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Target: 5'- ---aGGACCGcgcGGCuGAGguGGCGCu -3' miRNA: 3'- cguaCCUGGUuu-UCGcCUCuuCCGCG- -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 80873 | 0.68 | 0.961523 |
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Target: 5'- gGCAUcagcuGACCAAAgauagGGCGGGGAccuugggguccuAGGCGg -3' miRNA: 3'- -CGUAc----CUGGUUU-----UCGCCUCU------------UCCGCg -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 120047 | 0.68 | 0.961523 |
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Target: 5'- gGCGUuuuguucGGCCAaaacGAGGCGGGGGucgcgauuGGCGCu -3' miRNA: 3'- -CGUAc------CUGGU----UUUCGCCUCUu-------CCGCG- -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 65429 | 0.68 | 0.957808 |
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Target: 5'- -aGUGGAgCGuAGAGCGGGGc--GCGCg -3' miRNA: 3'- cgUACCUgGU-UUUCGCCUCuucCGCG- -5' |
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| 6197 | 5' | -51.9 | NC_001844.1 | + | 2352 | 1.15 | 0.002562 |
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Target: 5'- aGCAUGGACCAAAAGCGGAGAAGGCGCa -3' miRNA: 3'- -CGUACCUGGUUUUCGCCUCUUCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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