Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6198 | 3' | -52.8 | NC_001844.1 | + | 124878 | 0.66 | 0.973491 |
Target: 5'- aUGUCUgagUACAGGuccucauuaGCGGCuGCcCCCCCAc -3' miRNA: 3'- cGCAGA---AUGUCU---------UGUCG-UGuGGGGGU- -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 120374 | 0.66 | 0.967563 |
Target: 5'- aCGUCUgggcgccacgGCGGAgcGCAGaACGCUCCCGg -3' miRNA: 3'- cGCAGAa---------UGUCU--UGUCgUGUGGGGGU- -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 13765 | 0.67 | 0.953013 |
Target: 5'- uCGUCcucCAGAGCGG-GCACCUCCGg -3' miRNA: 3'- cGCAGaauGUCUUGUCgUGUGGGGGU- -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 1205 | 0.67 | 0.948783 |
Target: 5'- gGCuGUgUUGUGGAGguGCACACCCCa- -3' miRNA: 3'- -CG-CAgAAUGUCUUguCGUGUGGGGgu -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 144656 | 0.67 | 0.939583 |
Target: 5'- gGCGUCggGCuccAGCAGCGCgACCCUg- -3' miRNA: 3'- -CGCAGaaUGuc-UUGUCGUG-UGGGGgu -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 142443 | 0.68 | 0.934608 |
Target: 5'- cCGUCUUGCgccguggcccgAGGGCcGCGCACaCCCUg -3' miRNA: 3'- cGCAGAAUG-----------UCUUGuCGUGUG-GGGGu -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 25123 | 0.68 | 0.934608 |
Target: 5'- aGCGUCggggGC-GAGCaAGUcuACAUCCCCAu -3' miRNA: 3'- -CGCAGaa--UGuCUUG-UCG--UGUGGGGGU- -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 42753 | 0.68 | 0.927765 |
Target: 5'- cGCGcUCUggGCAGAgccgGCAagcgugaacguuguGCugGCCCCCGu -3' miRNA: 3'- -CGC-AGAa-UGUCU----UGU--------------CGugUGGGGGU- -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 21333 | 0.68 | 0.923903 |
Target: 5'- -aGUCaacaUGCGGAGUAGCAgGCCCCUg -3' miRNA: 3'- cgCAGa---AUGUCUUGUCGUgUGGGGGu -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 141518 | 0.69 | 0.885781 |
Target: 5'- aGCGUCUUGgcCAGAuuGGCGucccuaACCCCCu -3' miRNA: 3'- -CGCAGAAU--GUCUugUCGUg-----UGGGGGu -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 49662 | 0.69 | 0.885781 |
Target: 5'- aGCGc----CAGGuCGGCAUACCCCCAc -3' miRNA: 3'- -CGCagaauGUCUuGUCGUGUGGGGGU- -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 68403 | 0.69 | 0.878576 |
Target: 5'- cGCGUgcUGCAGAaagugcagauGCGGCaACGCCCCa- -3' miRNA: 3'- -CGCAgaAUGUCU----------UGUCG-UGUGGGGgu -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 86028 | 0.71 | 0.813122 |
Target: 5'- aCGUUUUgGCAGAuacuccCGGCGCACCCCgAg -3' miRNA: 3'- cGCAGAA-UGUCUu-----GUCGUGUGGGGgU- -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 120919 | 0.71 | 0.794859 |
Target: 5'- cCGcCUUugGGGACAGgGCGCCCgCGg -3' miRNA: 3'- cGCaGAAugUCUUGUCgUGUGGGgGU- -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 127119 | 0.73 | 0.706126 |
Target: 5'- cGCaGUCUUGgAGGccauGCAaCACACCCCCGg -3' miRNA: 3'- -CG-CAGAAUgUCU----UGUcGUGUGGGGGU- -5' |
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6198 | 3' | -52.8 | NC_001844.1 | + | 2627 | 1.11 | 0.004127 |
Target: 5'- uGCGUCUUACAGAACAGCACACCCCCAu -3' miRNA: 3'- -CGCAGAAUGUCUUGUCGUGUGGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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