Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6206 | 3' | -54 | NC_001844.1 | + | 107528 | 0.66 | 0.959002 |
Target: 5'- gGGGCuCCCCCAcAA-AAGCGaaaGCGg -3' miRNA: 3'- -CCCGcGGGGGUuUUaUUCGCgugUGC- -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 12707 | 0.66 | 0.959002 |
Target: 5'- cGGUGCCgCCGcacGAGUAgcuGGuCGCGCugGu -3' miRNA: 3'- cCCGCGGgGGU---UUUAU---UC-GCGUGugC- -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 54777 | 0.66 | 0.953592 |
Target: 5'- -aGCGCaCCCCAAAAguguacugugauGGCGCAgagUACGa -3' miRNA: 3'- ccCGCG-GGGGUUUUau----------UCGCGU---GUGC- -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 13891 | 0.66 | 0.950714 |
Target: 5'- -aGCGCUgCCGuacAGGUAaugcaauAGCGCACACa -3' miRNA: 3'- ccCGCGGgGGU---UUUAU-------UCGCGUGUGc -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 22837 | 0.66 | 0.947283 |
Target: 5'- gGGGCGUacuuggggcuuuguaCCUuc-GUAAGCGCGCAaCGg -3' miRNA: 3'- -CCCGCGg--------------GGGuuuUAUUCGCGUGU-GC- -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 19618 | 0.66 | 0.946843 |
Target: 5'- uGGGCGaCCCgCGcGAcuAGgGCGCAUGu -3' miRNA: 3'- -CCCGC-GGGgGUuUUauUCgCGUGUGC- -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 142726 | 0.66 | 0.946843 |
Target: 5'- aGGGUcagaGUCCCCGAGu--AGCcugGCACGCu -3' miRNA: 3'- -CCCG----CGGGGGUUUuauUCG---CGUGUGc -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 113598 | 0.67 | 0.932519 |
Target: 5'- aGGGCGCagCCCA----GAGCcacCGCGCGg -3' miRNA: 3'- -CCCGCGg-GGGUuuuaUUCGc--GUGUGC- -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 106273 | 0.67 | 0.927253 |
Target: 5'- aGGCGCCaaugCCCGuc-UGAGCGCgugguGCGCa -3' miRNA: 3'- cCCGCGG----GGGUuuuAUUCGCG-----UGUGc -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 113331 | 0.67 | 0.92174 |
Target: 5'- cGGU-CCUCCAc-AUGGGCGCGCugGa -3' miRNA: 3'- cCCGcGGGGGUuuUAUUCGCGUGugC- -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 47430 | 0.67 | 0.91598 |
Target: 5'- cGGCGCCcuacuCCCAAGc--GGUGCGCAUc -3' miRNA: 3'- cCCGCGG-----GGGUUUuauUCGCGUGUGc -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 144546 | 0.67 | 0.912407 |
Target: 5'- cGGGCGCagccaugccccaaggCCCCAucGGUuGGC-CGCGCGg -3' miRNA: 3'- -CCCGCG---------------GGGGUu-UUAuUCGcGUGUGC- -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 144770 | 0.67 | 0.909976 |
Target: 5'- -cGCGCCCauguggaggaCCGAGGUcGAGCGCugACc -3' miRNA: 3'- ccCGCGGG----------GGUUUUA-UUCGCGugUGc -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 112527 | 0.68 | 0.883546 |
Target: 5'- --aCGCCCCCuauuuGAAUGAGgGCcCGCGu -3' miRNA: 3'- cccGCGGGGGu----UUUAUUCgCGuGUGC- -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 67167 | 0.69 | 0.868935 |
Target: 5'- -aGCGCCUauaaaCGAAGUGcuAGcCGCACACGa -3' miRNA: 3'- ccCGCGGGg----GUUUUAU--UC-GCGUGUGC- -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 101325 | 0.69 | 0.868935 |
Target: 5'- cGGGCGCaaccguacaCCCAAAGUuguGGCGUAaGCu -3' miRNA: 3'- -CCCGCGg--------GGGUUUUAu--UCGCGUgUGc -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 120159 | 0.69 | 0.864378 |
Target: 5'- cGGCGCCCCCcgcuGUGAcaugcggcaggauccGuCGCACGCc -3' miRNA: 3'- cCCGCGGGGGuuu-UAUU---------------C-GCGUGUGc -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 141386 | 0.69 | 0.861297 |
Target: 5'- uGGUGCCCCUucucUGAGCGUcCGCu -3' miRNA: 3'- cCCGCGGGGGuuuuAUUCGCGuGUGc -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 142437 | 0.69 | 0.861297 |
Target: 5'- -uGCGCCguggCCCGAGGgccGCGCACACc -3' miRNA: 3'- ccCGCGG----GGGUUUUauuCGCGUGUGc -5' |
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6206 | 3' | -54 | NC_001844.1 | + | 142140 | 0.69 | 0.853446 |
Target: 5'- uGGCGUCCCCGAuGUGGG-GCAgGgGg -3' miRNA: 3'- cCCGCGGGGGUUuUAUUCgCGUgUgC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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