Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6359 | 3' | -56.4 | NC_001847.1 | + | 121127 | 1.08 | 0.002483 |
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Target: 5'- gCGCCUCUAUGCUGUCCAGCGAGAGGUa -3' miRNA: 3'- -GCGGAGAUACGACAGGUCGCUCUCCA- -5' |
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| 6359 | 3' | -56.4 | NC_001847.1 | + | 102096 | 0.68 | 0.775163 |
|
Target: 5'- gCGCCUCggccGCgagcgcGUCCAGCucggcgGAGAGGg -3' miRNA: 3'- -GCGGAGaua-CGa-----CAGGUCG------CUCUCCa -5' |
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| 6359 | 3' | -56.4 | NC_001847.1 | + | 98449 | 0.72 | 0.511887 |
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Target: 5'- cCGCCUCcaccacgccGCUcucGUCCAGCGGGGGGc -3' miRNA: 3'- -GCGGAGaua------CGA---CAGGUCGCUCUCCa -5' |
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| 6359 | 3' | -56.4 | NC_001847.1 | + | 89303 | 0.71 | 0.582563 |
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Target: 5'- gGCCUCggccGUGCcGcCCAGCGAGuGGc -3' miRNA: 3'- gCGGAGa---UACGaCaGGUCGCUCuCCa -5' |
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| 6359 | 3' | -56.4 | NC_001847.1 | + | 81056 | 0.69 | 0.685897 |
|
Target: 5'- gCGCCUCUAgGUUG-CCGGCGA-AGGc -3' miRNA: 3'- -GCGGAGAUaCGACaGGUCGCUcUCCa -5' |
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| 6359 | 3' | -56.4 | NC_001847.1 | + | 55071 | 0.7 | 0.644623 |
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Target: 5'- cCGCgUUUAUGCUGgcggCC-GCGGGGGGc -3' miRNA: 3'- -GCGgAGAUACGACa---GGuCGCUCUCCa -5' |
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| 6359 | 3' | -56.4 | NC_001847.1 | + | 18003 | 0.66 | 0.837625 |
|
Target: 5'- aGCgUCgggGCUGUUgGGCGuGGGGg -3' miRNA: 3'- gCGgAGauaCGACAGgUCGCuCUCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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