Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6515 | 5' | -51 | NC_001847.1 | + | 39808 | 0.66 | 0.990566 |
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Target: 5'- cCugGCUGCGCCCccUGCGcCUUCGg--- -3' miRNA: 3'- -GugUGAUGUGGGc-ACGU-GAAGCaacc -5' |
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| 6515 | 5' | -51 | NC_001847.1 | + | 94683 | 0.66 | 0.991062 |
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Target: 5'- gACGCuUAUGCCCGUGCcgcgcaaguacuggcGCUUCagcagcgaGUUGGc -3' miRNA: 3'- gUGUG-AUGUGGGCACG---------------UGAAG--------CAACC- -5' |
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| 6515 | 5' | -51 | NC_001847.1 | + | 104050 | 0.66 | 0.991768 |
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Target: 5'- cCGCGCUGCccgGCCCcaGCGCcgcgUCGUacUGGu -3' miRNA: 3'- -GUGUGAUG---UGGGcaCGUGa---AGCA--ACC- -5' |
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| 6515 | 5' | -51 | NC_001847.1 | + | 30145 | 0.66 | 0.991768 |
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Target: 5'- aCGCGCgGCGCgCCGUGCGCccugggugCG-UGGc -3' miRNA: 3'- -GUGUGaUGUG-GGCACGUGaa------GCaACC- -5' |
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| 6515 | 5' | -51 | NC_001847.1 | + | 23521 | 0.66 | 0.992846 |
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Target: 5'- gCACACgaggucgcCGCCCGUGaagACgccgUUGUUGGu -3' miRNA: 3'- -GUGUGau------GUGGGCACg--UGa---AGCAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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