miRNA: 3'- gUGUGaUG--UGGGCACGUGaaGCaaCC- -5'
miRNA: 3'- gUGUGau--GUGGGCA---CGUGAAGCAaCC- -5'
miRNA: 3'- -GUGugAUGUGGGC----ACG---UGAAGCaACC- -5'
miRNA: 3'- gUGUGAUGUGGGC--------------ACGUGAag---CAACC- -5'
miRNA: 3'- -GUGUGAUGUGGGCACGUGAAGCAACC- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6515 | 5' | -51 | NC_001847.1 | + | 72021 | 0.71 | 0.896562 |
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Target: 5'- uGCACaACcgACCCGUGCGCggCGgcGGc -3' miRNA: 3'- gUGUGaUG--UGGGCACGUGaaGCaaCC- -5' |
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| 6515 | 5' | -51 | NC_001847.1 | + | 72624 | 0.72 | 0.867577 |
|
Target: 5'- gGCACgugcCGCCCGUgccGCACUUUGU-GGg -3' miRNA: 3'- gUGUGau--GUGGGCA---CGUGAAGCAaCC- -5' |
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| 6515 | 5' | -51 | NC_001847.1 | + | 23150 | 0.75 | 0.710226 |
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Target: 5'- gCGCugUACACCCGcgagUGCaccACUUCGaUGGa -3' miRNA: 3'- -GUGugAUGUGGGC----ACG---UGAAGCaACC- -5' |
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| 6515 | 5' | -51 | NC_001847.1 | + | 43149 | 0.78 | 0.52874 |
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Target: 5'- gGCGCUGCGCCCGcagucgcuggcgacUGCGCUgcugaGUUGGa -3' miRNA: 3'- gUGUGAUGUGGGC--------------ACGUGAag---CAACC- -5' |
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| 6515 | 5' | -51 | NC_001847.1 | + | 656 | 1.1 | 0.006894 |
|
Target: 5'- cCACACUACACCCGUGCACUUCGUUGGc -3' miRNA: 3'- -GUGUGAUGUGGGCACGUGAAGCAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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