Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6535 | 3' | -53.9 | NC_001847.1 | + | 82962 | 0.71 | 0.675749 |
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Target: 5'- gGCCGCCUGCgaucuguGGUGgCUGCUcCGGCGg -3' miRNA: 3'- gUGGUGGACG-------UCAUgGACGA-GUUGUa -5' |
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| 6535 | 3' | -53.9 | NC_001847.1 | + | 88631 | 0.71 | 0.655517 |
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Target: 5'- gCGCgCACgUGCgcgAGaGCCUGCUCAGCGUg -3' miRNA: 3'- -GUG-GUGgACG---UCaUGGACGAGUUGUA- -5' |
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| 6535 | 3' | -53.9 | NC_001847.1 | + | 10821 | 0.72 | 0.644835 |
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Target: 5'- gCGCgCugCUGCAGcACCUGCgcgCGACGg -3' miRNA: 3'- -GUG-GugGACGUCaUGGACGa--GUUGUa -5' |
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| 6535 | 3' | -53.9 | NC_001847.1 | + | 62254 | 0.72 | 0.644835 |
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Target: 5'- gCGCCACCUGCAG---CUGCcCGACAa -3' miRNA: 3'- -GUGGUGGACGUCaugGACGaGUUGUa -5' |
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| 6535 | 3' | -53.9 | NC_001847.1 | + | 106095 | 0.72 | 0.644835 |
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Target: 5'- cCACCGCCUGCAGcACguCUGC-CGGCGc -3' miRNA: 3'- -GUGGUGGACGUCaUG--GACGaGUUGUa -5' |
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| 6535 | 3' | -53.9 | NC_001847.1 | + | 131027 | 0.73 | 0.570249 |
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Target: 5'- gACCGCCUGU-GUGCCUcGCUCAuccaGCAg -3' miRNA: 3'- gUGGUGGACGuCAUGGA-CGAGU----UGUa -5' |
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| 6535 | 3' | -53.9 | NC_001847.1 | + | 60559 | 0.8 | 0.23796 |
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Target: 5'- aCGCCGuCCUGCAGgcCCUGCgUCAGCAg -3' miRNA: 3'- -GUGGU-GGACGUCauGGACG-AGUUGUa -5' |
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| 6535 | 3' | -53.9 | NC_001847.1 | + | 15252 | 1.06 | 0.004628 |
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Target: 5'- cCACCACCUGCAGUACCUGCUCAACAUg -3' miRNA: 3'- -GUGGUGGACGUCAUGGACGAGUUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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