Results 1 - 20 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6684 | 5' | -55 | NC_001847.1 | + | 26691 | 0.72 | 0.662088 |
Target: 5'- gCCGGcGGGCUCCAgccgcagcUCGgcGUCCAcgUCCa -3' miRNA: 3'- -GGCCuUCCGAGGU--------AGCa-CAGGU--AGGg -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 101883 | 0.66 | 0.928631 |
Target: 5'- ---aGGGGCUCCAUCGcGUcggCCAgCCCg -3' miRNA: 3'- ggccUUCCGAGGUAGCaCA---GGUaGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 40989 | 0.66 | 0.921068 |
Target: 5'- gCCGGGccgcgcucaccuGCUCCggcGUCGUG-CCGUCCUg -3' miRNA: 3'- -GGCCUuc----------CGAGG---UAGCACaGGUAGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 102895 | 0.67 | 0.917684 |
Target: 5'- cCCGGGcccgcucuGGGCUCCGccccuggGUCCGgcgCCCc -3' miRNA: 3'- -GGCCU--------UCCGAGGUagca---CAGGUa--GGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 2620 | 0.67 | 0.911853 |
Target: 5'- gCCGGAGGuGCUUCggCGggaccgCCGUCgCCg -3' miRNA: 3'- -GGCCUUC-CGAGGuaGCaca---GGUAG-GG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 60995 | 0.67 | 0.911257 |
Target: 5'- aCGGcgccgucGAGGCcgCCAUCGgcgccgcgcGUCaCAUCCCc -3' miRNA: 3'- gGCC-------UUCCGa-GGUAGCa--------CAG-GUAGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 120260 | 0.67 | 0.905787 |
Target: 5'- gCGGAcGGCcCCGuagcguUCGcUGUCCA-CCCg -3' miRNA: 3'- gGCCUuCCGaGGU------AGC-ACAGGUaGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 129209 | 0.67 | 0.905787 |
Target: 5'- gCGGGcGGGC-CCGUCGUccgccGcCCAUCCg -3' miRNA: 3'- gGCCU-UCCGaGGUAGCA-----CaGGUAGGg -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 55285 | 0.67 | 0.905167 |
Target: 5'- cCCGGAcGGGCggcaGUCGUGUCCcagcggcGUCUa -3' miRNA: 3'- -GGCCU-UCCGagg-UAGCACAGG-------UAGGg -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 108978 | 0.68 | 0.886194 |
Target: 5'- -aGGGAGGCgcggCaAUUGUGUCCAggCUCa -3' miRNA: 3'- ggCCUUCCGa---GgUAGCACAGGUa-GGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 108884 | 0.68 | 0.886194 |
Target: 5'- -aGGGAGGCgcggCaAUUGUGUCCAggCUCa -3' miRNA: 3'- ggCCUUCCGa---GgUAGCACAGGUa-GGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 103133 | 0.68 | 0.87921 |
Target: 5'- gCCGGAGcuucGcGCUCCAcgccCGcGUCCcUCCCg -3' miRNA: 3'- -GGCCUU----C-CGAGGUa---GCaCAGGuAGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 63694 | 0.68 | 0.864589 |
Target: 5'- cCCGGAAGcGC-CCggggccaaccucGUCGUGUgCAcccUCCCc -3' miRNA: 3'- -GGCCUUC-CGaGG------------UAGCACAgGU---AGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 76617 | 0.68 | 0.856963 |
Target: 5'- aCCGcGgcGGcCUCU-UCGUGUCCcugCCCg -3' miRNA: 3'- -GGC-CuuCC-GAGGuAGCACAGGua-GGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 105255 | 0.68 | 0.849133 |
Target: 5'- gCCGGcAGGGCcgCCGccUCGccGUCCccGUCCCc -3' miRNA: 3'- -GGCC-UUCCGa-GGU--AGCa-CAGG--UAGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 27149 | 0.68 | 0.849133 |
Target: 5'- uCCGGggGGCgcagCCG-CGgcggCCAgCCCa -3' miRNA: 3'- -GGCCuuCCGa---GGUaGCaca-GGUaGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 5368 | 0.69 | 0.832892 |
Target: 5'- gCCGGGcccAGGCUCCggCG-GUCCGagcggCCg -3' miRNA: 3'- -GGCCU---UCCGAGGuaGCaCAGGUa----GGg -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 92384 | 0.69 | 0.824494 |
Target: 5'- cUCGGAAGGCcgUCCuggAUCugcUGUCCGcCCCg -3' miRNA: 3'- -GGCCUUCCG--AGG---UAGc--ACAGGUaGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 96487 | 0.7 | 0.751732 |
Target: 5'- gCGcGAGGGCUCCGuguUCGuUGUCCcgUUCg -3' miRNA: 3'- gGC-CUUCCGAGGU---AGC-ACAGGuaGGG- -5' |
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6684 | 5' | -55 | NC_001847.1 | + | 123502 | 1.13 | 0.002147 |
Target: 5'- gCCGGAAGGCUCCAUCGUGUCCAUCCCc -3' miRNA: 3'- -GGCCUUCCGAGGUAGCACAGGUAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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