Results 1 - 20 of 182 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6687 | 5' | -60.5 | NC_001847.1 | + | 265 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 102913 | 0.66 | 0.720985 |
Target: 5'- uCCgCCCCUgggUCCGGCgCCCCGCg---- -3' miRNA: 3'- -GGgGGGGG---GGGUUGaGGGGCGaaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 100492 | 0.66 | 0.720985 |
Target: 5'- gCCgCCCCCUCGGCgCgCCGCUc--- -3' miRNA: 3'- gGGgGGGGGGGUUGaGgGGCGAaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 77991 | 0.66 | 0.720985 |
Target: 5'- gCCCCUCgCCCAGCUCgUgGCa---- -3' miRNA: 3'- gGGGGGGgGGGUUGAGgGgCGaaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 100 | 0.66 | 0.720985 |
Target: 5'- uCCgCCCCUgggUCCGGCgCCCCGCg---- -3' miRNA: 3'- -GGgGGGGG---GGGUUGaGGGGCGaaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 105461 | 0.66 | 0.720985 |
Target: 5'- uUCgUCCUCCCCGucCUCCCCGUc---- -3' miRNA: 3'- -GGgGGGGGGGGUu-GAGGGGCGaaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 6012 | 0.66 | 0.720985 |
Target: 5'- gCCgCCCCUCaCCCuuCUCCCuuuCGCgaUUGg -3' miRNA: 3'- -GG-GGGGGG-GGGuuGAGGG---GCGaaAAC- -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 108825 | 0.66 | 0.720985 |
Target: 5'- gCCgCCCCUCaCCCuuCUCCCuuuCGCgaUUGg -3' miRNA: 3'- -GG-GGGGGG-GGGuuGAGGG---GCGaaAAC- -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 405 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 2648 | 0.66 | 0.720985 |
Target: 5'- uUCgUCCUCCCCGucCUCCCCGUc---- -3' miRNA: 3'- -GGgGGGGGGGGUu-GAGGGGCGaaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 377 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 349 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 237 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 321 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 293 | 0.66 | 0.720985 |
Target: 5'- uCCUCCUCCCUCGcuccucCUCCCuCGCUc--- -3' miRNA: 3'- -GGGGGGGGGGGUu-----GAGGG-GCGAaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 40239 | 0.66 | 0.711312 |
Target: 5'- aCCCCCagCCCCGGCUggCCCGUg---- -3' miRNA: 3'- gGGGGGg-GGGGUUGAg-GGGCGaaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 36406 | 0.66 | 0.711312 |
Target: 5'- cCCCggCCCCggCCCCGGC-CCCgGCUUc-- -3' miRNA: 3'- -GGG--GGGG--GGGGUUGaGGGgCGAAaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 73826 | 0.66 | 0.711312 |
Target: 5'- gCCCgCCCCgCCCCG-CgcaCCCGCg---- -3' miRNA: 3'- -GGG-GGGG-GGGGUuGag-GGGCGaaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 124817 | 0.66 | 0.701574 |
Target: 5'- uCCUCCCCCUCCucguccgcCUCCUCGUc---- -3' miRNA: 3'- -GGGGGGGGGGGuu------GAGGGGCGaaaac -5' |
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6687 | 5' | -60.5 | NC_001847.1 | + | 63728 | 0.66 | 0.701574 |
Target: 5'- aCCCUCCCCCCGGCcgagcaacagCgCCGCc---- -3' miRNA: 3'- gGGGGGGGGGGUUGa---------GgGGCGaaaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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