Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 673 | 3' | -62.1 | AC_000018.1 | + | 8493 | 0.66 | 0.25294 |
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Target: 5'- cGGCGcc-GCGCGCGGGUAgGUuCUGg -3' miRNA: 3'- cCCGCucaCGCGUGCCCGUgCGcGAC- -5' |
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| 673 | 3' | -62.1 | AC_000018.1 | + | 15736 | 0.68 | 0.195129 |
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Target: 5'- cGGCGucugGCGUcaguaACGGuCACGCGCUGg -3' miRNA: 3'- cCCGCuca-CGCG-----UGCCcGUGCGCGAC- -5' |
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| 673 | 3' | -62.1 | AC_000018.1 | + | 10979 | 0.69 | 0.157529 |
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Target: 5'- aGGGCGAGggacugGCGCGCcuGGGCGCaccauCGCc- -3' miRNA: 3'- -CCCGCUCa-----CGCGUG--CCCGUGc----GCGac -5' |
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| 673 | 3' | -62.1 | AC_000018.1 | + | 11251 | 0.69 | 0.157529 |
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Target: 5'- aGGGCGAGggacugGCGCGCcuGGGCGCaccauCGCc- -3' miRNA: 3'- -CCCGCUCa-----CGCGUG--CCCGUGc----GCGac -5' |
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| 673 | 3' | -62.1 | AC_000018.1 | + | 16576 | 0.7 | 0.153318 |
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Target: 5'- cGGCGcGUGCagugGCGCGGGCGCaaaGUGCg- -3' miRNA: 3'- cCCGCuCACG----CGUGCCCGUG---CGCGac -5' |
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| 673 | 3' | -62.1 | AC_000018.1 | + | 11324 | 0.7 | 0.149209 |
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Target: 5'- cGGGCGAugGUGCGCcCaGGCGCGC-Ca- -3' miRNA: 3'- -CCCGCU--CACGCGuGcCCGUGCGcGac -5' |
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| 673 | 3' | -62.1 | AC_000018.1 | + | 11067 | 0.76 | 0.049391 |
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Target: 5'- uGGGCGGGUGcCGCuCGGGCgauggugcgcccagGCGCGCc- -3' miRNA: 3'- -CCCGCUCAC-GCGuGCCCG--------------UGCGCGac -5' |
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| 673 | 3' | -62.1 | AC_000018.1 | + | 16382 | 1.1 | 0.000104 |
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Target: 5'- gGGGCGAGUGCGCACGGGCACGCGCUGg -3' miRNA: 3'- -CCCGCUCACGCGUGCCCGUGCGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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