miRNA: 3'- -GACUGCUCGUGCaa-CGCCGaguuuuu -5'
miRNA: 3'- -GACUGCUCGUgcaaCGCCGAGUuuuu -5'
miRNA: 3'- -GACUGCUCGUGCAACGc-CGa--GUUUUu -5'
miRNA: 3'- gaCUGC--UCGUGCaACGCCGAGUUUuu -5'
miRNA: 3'- -GACUGCUCGUGCAACGCCGAGUUUUU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6731 | 5' | -51 | NC_001875.2 | + | 129186 | 0.72 | 0.781391 |
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Target: 5'- uCUGGCGAGCGCGagcGCGGCg------ -3' miRNA: 3'- -GACUGCUCGUGCaa-CGCCGaguuuuu -5' |
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| 6731 | 5' | -51 | NC_001875.2 | + | 59711 | 0.73 | 0.771508 |
|
Target: 5'- gCUGGCGAGCGagcaGCGGCUCGc--- -3' miRNA: 3'- -GACUGCUCGUgcaaCGCCGAGUuuuu -5' |
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| 6731 | 5' | -51 | NC_001875.2 | + | 121985 | 0.74 | 0.688326 |
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Target: 5'- gCUGGCGGGCACGUUGCccGCcagCGAAAu -3' miRNA: 3'- -GACUGCUCGUGCAACGc-CGa--GUUUUu -5' |
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| 6731 | 5' | -51 | NC_001875.2 | + | 95553 | 0.79 | 0.446407 |
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Target: 5'- -cGACGccGGCGCGcUGCGGCUCGAAu- -3' miRNA: 3'- gaCUGC--UCGUGCaACGCCGAGUUUuu -5' |
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| 6731 | 5' | -51 | NC_001875.2 | + | 5316 | 1.06 | 0.009448 |
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Target: 5'- gCUGACGAGCACGUUGCGGCUCAAAAAc -3' miRNA: 3'- -GACUGCUCGUGCAACGCCGAGUUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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