Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
6734 | 5' | -57.6 | NC_001875.2 | + | 63728 | 0.68 | 0.672561 |
Target: 5'- cGACgGCGGGCgcuUCGCcgGCGUCGCu- -3' miRNA: 3'- aCUGaCGUCCG---GGCGaaCGCAGUGuu -5' |
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6734 | 5' | -57.6 | NC_001875.2 | + | 110896 | 0.67 | 0.703524 |
Target: 5'- -cGCUGgAcGGCCCGCggGCGUUugAGc -3' miRNA: 3'- acUGACgU-CCGGGCGaaCGCAGugUU- -5' |
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6734 | 5' | -57.6 | NC_001875.2 | + | 129523 | 0.66 | 0.743882 |
Target: 5'- -aGCUGCAGGCCgaGCUggccgagcUGCG-CGCGg -3' miRNA: 3'- acUGACGUCCGGg-CGA--------ACGCaGUGUu -5' |
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6734 | 5' | -57.6 | NC_001875.2 | + | 39165 | 0.66 | 0.753741 |
Target: 5'- gGGCcgcgagGCGGGCCgGCgguUGCGUgGCGu -3' miRNA: 3'- aCUGa-----CGUCCGGgCGa--ACGCAgUGUu -5' |
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6734 | 5' | -57.6 | NC_001875.2 | + | 16237 | 0.73 | 0.361411 |
Target: 5'- gUGACUGCggcgacgAGGCCgucaCGCgcgUGCGUCACGu -3' miRNA: 3'- -ACUGACG-------UCCGG----GCGa--ACGCAGUGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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