miRNA: 3'- gaCGCGGaa------ACGaa--CGUUaUGGCGGa -5'
miRNA: 3'- gaCGCGgaaACGAaCGUUAuGGCGGa -5'
miRNA: 3'- gaC-GCGGaaACGA--ACGUUa--UGGCGGa -5'
miRNA: 3'- gaC-GCGGaa-ACGAACGuua----UGGCGGa -5'
miRNA: 3'- -GACGCGGAAACGAACGUUAUGGCGGA- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6744 | 3' | -52.8 | NC_001875.2 | + | 86928 | 0.68 | 0.87301 |
|
Target: 5'- -aGCGCCgccuagagUGCgaggGCGA-GCCGCCa -3' miRNA: 3'- gaCGCGGaa------ACGaa--CGUUaUGGCGGa -5' |
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| 6744 | 3' | -52.8 | NC_001875.2 | + | 79107 | 0.68 | 0.887835 |
|
Target: 5'- -cGCGCaaaUGCUgGCGAU-CCGCCg -3' miRNA: 3'- gaCGCGgaaACGAaCGUUAuGGCGGa -5' |
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| 6744 | 3' | -52.8 | NC_001875.2 | + | 119874 | 0.68 | 0.894877 |
|
Target: 5'- -cGuCGCCagUGCUucUGCAAcgcGCCGCCg -3' miRNA: 3'- gaC-GCGGaaACGA--ACGUUa--UGGCGGa -5' |
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| 6744 | 3' | -52.8 | NC_001875.2 | + | 114721 | 0.68 | 0.894877 |
|
Target: 5'- -cGaCGCCaaaUGCUUGCuuaugcgGCCGCCa -3' miRNA: 3'- gaC-GCGGaa-ACGAACGuua----UGGCGGa -5' |
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| 6744 | 3' | -52.8 | NC_001875.2 | + | 113231 | 1.09 | 0.004212 |
|
Target: 5'- gCUGCGCCUUUGCUUGCAAUACCGCCUg -3' miRNA: 3'- -GACGCGGAAACGAACGUUAUGGCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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