Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
675 | 3' | -57.2 | AC_000018.1 | + | 22056 | 0.67 | 0.457733 |
Target: 5'- uAGCGC-CUCCUGGuugcgGCGCaGggUGGGc -3' miRNA: 3'- cUCGUGuGGGGGCU-----UGCGcCuuACCC- -5' |
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675 | 3' | -57.2 | AC_000018.1 | + | 28189 | 0.67 | 0.447737 |
Target: 5'- gGAGCuCACCUCCGGuuuUG-GGAGUGGu -3' miRNA: 3'- -CUCGuGUGGGGGCUu--GCgCCUUACCc -5' |
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675 | 3' | -57.2 | AC_000018.1 | + | 17301 | 0.67 | 0.428115 |
Target: 5'- uAGCGaguGCCUCUGuAACcCGGAGUGGGa -3' miRNA: 3'- cUCGUg--UGGGGGC-UUGcGCCUUACCC- -5' |
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675 | 3' | -57.2 | AC_000018.1 | + | 5064 | 0.68 | 0.372443 |
Target: 5'- cAGCAgACCUCCucguuuCGCGGGuUGGGa -3' miRNA: 3'- cUCGUgUGGGGGcuu---GCGCCUuACCC- -5' |
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675 | 3' | -57.2 | AC_000018.1 | + | 21628 | 0.72 | 0.201606 |
Target: 5'- uAGUcaACACUgCCGGACGCGaGAcgGGGg -3' miRNA: 3'- cUCG--UGUGGgGGCUUGCGC-CUuaCCC- -5' |
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675 | 3' | -57.2 | AC_000018.1 | + | 18492 | 0.75 | 0.140879 |
Target: 5'- cGGGCGCgguguGCCCCCaGGACGCGGucUGGc -3' miRNA: 3'- -CUCGUG-----UGGGGG-CUUGCGCCuuACCc -5' |
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675 | 3' | -57.2 | AC_000018.1 | + | 15957 | 0.75 | 0.133173 |
Target: 5'- uGAGUGCGCCCCaggGAGCGCGGAAa--- -3' miRNA: 3'- -CUCGUGUGGGGg--CUUGCGCCUUaccc -5' |
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675 | 3' | -57.2 | AC_000018.1 | + | 23748 | 1.09 | 0.0004 |
Target: 5'- gGAGCACACCCCCGAACGCGGAAUGGGc -3' miRNA: 3'- -CUCGUGUGGGGGCUUGCGCCUUACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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