Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
676 | 3' | -56.4 | AC_000018.1 | + | 10691 | 0.66 | 0.494309 |
Target: 5'- --gGGCGCGAGcCACUCcCAGCcCCGa- -3' miRNA: 3'- ucgCCGCGUUU-GUGAG-GUCGaGGUgu -5' |
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676 | 3' | -56.4 | AC_000018.1 | + | 17681 | 0.67 | 0.432108 |
Target: 5'- uGGCGGUuCAGGC-CUCgCAGCgCCACu -3' miRNA: 3'- -UCGCCGcGUUUGuGAG-GUCGaGGUGu -5' |
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676 | 3' | -56.4 | AC_000018.1 | + | 12321 | 0.67 | 0.402766 |
Target: 5'- gGGCGGCGCu-GCAgagCCAGCcgUCCGgCAu -3' miRNA: 3'- -UCGCCGCGuuUGUga-GGUCG--AGGU-GU- -5' |
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676 | 3' | -56.4 | AC_000018.1 | + | 8537 | 0.68 | 0.39327 |
Target: 5'- cGCGGCGCcGACguaccACUgCCAGCcaugCCGCc -3' miRNA: 3'- uCGCCGCGuUUG-----UGA-GGUCGa---GGUGu -5' |
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676 | 3' | -56.4 | AC_000018.1 | + | 16181 | 0.68 | 0.365669 |
Target: 5'- gAGCGGC-CAGACGCg-CGGCUUCAg- -3' miRNA: 3'- -UCGCCGcGUUUGUGagGUCGAGGUgu -5' |
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676 | 3' | -56.4 | AC_000018.1 | + | 21199 | 0.69 | 0.306621 |
Target: 5'- uGUGGCGCAuACcguuCUCCAGCaacuucaugUCCAUg -3' miRNA: 3'- uCGCCGCGUuUGu---GAGGUCG---------AGGUGu -5' |
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676 | 3' | -56.4 | AC_000018.1 | + | 25616 | 0.69 | 0.306621 |
Target: 5'- cAGCGGCaguGGCAUUCCAGCagaccgucUCCGCu -3' miRNA: 3'- -UCGCCGcguUUGUGAGGUCG--------AGGUGu -5' |
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676 | 3' | -56.4 | AC_000018.1 | + | 24957 | 1.09 | 0.00038 |
Target: 5'- aAGCGGCGCAAACACUCCAGCUCCACAg -3' miRNA: 3'- -UCGCCGCGUUUGUGAGGUCGAGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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