miRNA: 3'- gaGCUUGUCG--GC-------------UAGCUUGAGCCGCU- -5'
miRNA: 3'- -GAGCUUGU-CGGCuAGCu--UGaGCCGCU- -5'
miRNA: 3'- -GAGCUUGU-CGGCUAGCu-----UGAGCCGCU- -5'
miRNA: 3'- -GAGCUUGUCGGCUAGCUUGAGCCGCU- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 6760 | 5' | -53.6 | NC_001875.2 | + | 119464 | 0.72 | 0.747096 |
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Target: 5'- -aCGAGCGGCacCGuggcacccucgucGUCGGGCUUGGCGAa -3' miRNA: 3'- gaGCUUGUCG--GC-------------UAGCUUGAGCCGCU- -5' |
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| 6760 | 5' | -53.6 | NC_001875.2 | + | 437 | 0.72 | 0.708096 |
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Target: 5'- aCUCGAGCGuGCCG-UCGugcAC-CGGCGAg -3' miRNA: 3'- -GAGCUUGU-CGGCuAGCu--UGaGCCGCU- -5' |
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| 6760 | 5' | -53.6 | NC_001875.2 | + | 103100 | 0.74 | 0.635927 |
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Target: 5'- uCUUGGACAcGUCGAUCGuggcucGCUUGGCGGc -3' miRNA: 3'- -GAGCUUGU-CGGCUAGCu-----UGAGCCGCU- -5' |
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| 6760 | 5' | -53.6 | NC_001875.2 | + | 96828 | 1.1 | 0.004322 |
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Target: 5'- cCUCGAACAGCCGAUCGAACUCGGCGAg -3' miRNA: 3'- -GAGCUUGUCGGCUAGCUUGAGCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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