Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 6783 | 5' | -49.9 | NC_001875.2 | + | 117881 | 0.69 | 0.948897 |
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Target: 5'- aGCGuuGGCCAGCuuaGGCuGUUGUUUg-- -3' miRNA: 3'- gCGUuuCCGGUCG---CCG-UAACAAAagu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 101791 | 0.69 | 0.944252 |
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Target: 5'- uGCAAAGGCCGGCaagcaauuggccGGCGUUuacggUCGa -3' miRNA: 3'- gCGUUUCCGGUCG------------CCGUAAcaaa-AGU- -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 107848 | 0.7 | 0.939339 |
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Target: 5'- uCGCGggcGAGGCCAGCGGCc--------- -3' miRNA: 3'- -GCGU---UUCCGGUCGCCGuaacaaaagu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 131746 | 0.7 | 0.934157 |
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Target: 5'- uGCAuuGGGCCGGCGGCGg-------- -3' miRNA: 3'- gCGUu-UCCGGUCGCCGUaacaaaagu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 36341 | 0.7 | 0.928702 |
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Target: 5'- gCGCGuguacgcGGCCGGCGGCGacuUUGUcgUCu -3' miRNA: 3'- -GCGUuu-----CCGGUCGCCGU---AACAaaAGu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 124769 | 0.71 | 0.890296 |
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Target: 5'- gCGCAcAGGCCGGUGGCGUc------- -3' miRNA: 3'- -GCGUuUCCGGUCGCCGUAacaaaagu -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 121449 | 0.72 | 0.875389 |
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Target: 5'- cCGCGccacGGCgCGGCGGCGUUcaaaGUUUUCGg -3' miRNA: 3'- -GCGUuu--CCG-GUCGCCGUAA----CAAAAGU- -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 79550 | 1.1 | 0.007149 |
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Target: 5'- gCGCAAAGGCCAGCGGCAUUGUUUUCAa -3' miRNA: 3'- -GCGUUUCCGGUCGCCGUAACAAAAGU- -5' |
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| 6783 | 5' | -49.9 | NC_001875.2 | + | 43903 | 0.66 | 0.993748 |
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Target: 5'- uGCAGAGGCggggcguuugCGGUGGCAUcugGUaUUCc -3' miRNA: 3'- gCGUUUCCG----------GUCGCCGUAa--CAaAAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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