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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 691 | 3' | -55.7 | AC_000018.1 | + | 21502 | 1.07 | 0.000555 |
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Target: 5'- gAAACGGCUCCAGCGAGCAAGAGCUCAa -3' miRNA: 3'- -UUUGCCGAGGUCGCUCGUUCUCGAGU- -5' |
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| 691 | 3' | -55.7 | AC_000018.1 | + | 12673 | 0.69 | 0.332543 |
|
Target: 5'- cGAGCGGUUCCAGCGcGGCGccaacuuGGGgUCGu -3' miRNA: 3'- -UUUGCCGAGGUCGC-UCGUu------CUCgAGU- -5' |
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| 691 | 3' | -55.7 | AC_000018.1 | + | 3527 | 0.68 | 0.367851 |
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Target: 5'- cGGGCaGGCUCCcaccaUGGGCAGGAGUUCGu -3' miRNA: 3'- -UUUG-CCGAGGuc---GCUCGUUCUCGAGU- -5' |
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| 691 | 3' | -55.7 | AC_000018.1 | + | 5090 | 0.67 | 0.445804 |
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Target: 5'- gGGACGGCUCCuG-GAGUAGGGaaUCAg -3' miRNA: 3'- -UUUGCCGAGGuCgCUCGUUCUcgAGU- -5' |
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| 691 | 3' | -55.7 | AC_000018.1 | + | 9257 | 0.66 | 0.477328 |
|
Target: 5'- -cGCGGCgacgCCGGCGGcGCAcGGGCa-- -3' miRNA: 3'- uuUGCCGa---GGUCGCU-CGUuCUCGagu -5' |
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| 691 | 3' | -55.7 | AC_000018.1 | + | 16115 | 0.66 | 0.498945 |
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Target: 5'- --uCGGCucuUCCGGCGAGCAuaGGCg-- -3' miRNA: 3'- uuuGCCG---AGGUCGCUCGUucUCGagu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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