Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 7048 | 5' | -56.4 | NC_001876.1 | + | 16217 | 0.66 | 0.511474 |
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Target: 5'- cGGGCAGgCgCACaCCCGAGCU-CGuGUa -3' miRNA: 3'- -CUCGUUgGgGUG-GGGCUCGAuGUuCG- -5' |
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| 7048 | 5' | -56.4 | NC_001876.1 | + | 34286 | 0.66 | 0.500683 |
|
Target: 5'- -cGCGacgcGCUCCGCCCCacAGUUACGucAGCa -3' miRNA: 3'- cuCGU----UGGGGUGGGGc-UCGAUGU--UCG- -5' |
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| 7048 | 5' | -56.4 | NC_001876.1 | + | 26850 | 0.66 | 0.468943 |
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Target: 5'- aAGCugacguCCCUACCUgucaCGAGCccgGCAAGCc -3' miRNA: 3'- cUCGuu----GGGGUGGG----GCUCGa--UGUUCG- -5' |
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| 7048 | 5' | -56.4 | NC_001876.1 | + | 11161 | 0.66 | 0.468943 |
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Target: 5'- aAGCcugGGCCCCGCCCCuuccaauccuAGuUUGCAGGCu -3' miRNA: 3'- cUCG---UUGGGGUGGGGc---------UC-GAUGUUCG- -5' |
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| 7048 | 5' | -56.4 | NC_001876.1 | + | 11289 | 0.66 | 0.500683 |
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Target: 5'- -cGCAGCCCCAggcgaaCCG-GUUugGAGCc -3' miRNA: 3'- cuCGUUGGGGUgg----GGCuCGAugUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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