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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7070 | 5' | -59.6 | NC_001885.1 | + | 790 | 0.66 | 0.11849 |
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Target: 5'- uGGccACCCCACgCCGaauGuGGGCCACuCGGa -3' miRNA: 3'- gCCuuUGGGGUG-GGU---C-CCUGGUG-GCU- -5' |
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| 7070 | 5' | -59.6 | NC_001885.1 | + | 3506 | 0.66 | 0.11849 |
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Target: 5'- gGGAAGCaa-ACCCAGGGAUCcagaGCCu- -3' miRNA: 3'- gCCUUUGgggUGGGUCCCUGG----UGGcu -5' |
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| 7070 | 5' | -59.6 | NC_001885.1 | + | 2199 | 0.66 | 0.114833 |
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Target: 5'- aGGGuucgGACCCCcuccccgaACCUAGGGuaaCACUGAc -3' miRNA: 3'- gCCU----UUGGGG--------UGGGUCCCug-GUGGCU- -5' |
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| 7070 | 5' | -59.6 | NC_001885.1 | + | 6555 | 0.76 | 0.016222 |
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Target: 5'- aGGggGCCuuccuaaCCuuaaaugcuaccaACCCAGGGGCCACUGAg -3' miRNA: 3'- gCCuuUGG-------GG-------------UGGGUCCCUGGUGGCU- -5' |
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| 7070 | 5' | -59.6 | NC_001885.1 | + | 163 | 1.08 | 0.000028 |
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Target: 5'- uCGGAAACCCCACCCAGGGACCACCGAc -3' miRNA: 3'- -GCCUUUGGGGUGGGUCCCUGGUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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