Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7112 | 3' | -52.6 | NC_001895.1 | + | 9263 | 0.66 | 0.744352 |
Target: 5'- ---aCUGAUGcacACUGGCGAUuuUCGGCg -3' miRNA: 3'- agugGACUAU---UGGCCGCUAugGGUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 2128 | 0.66 | 0.744352 |
Target: 5'- ---aCUGuuUAACCGGgGAcGCgCCAGCg -3' miRNA: 3'- agugGACu-AUUGGCCgCUaUG-GGUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 11077 | 0.66 | 0.744352 |
Target: 5'- aUCACCcgUGAgccGACCGGUG-UGCgCAGg -3' miRNA: 3'- -AGUGG--ACUa--UUGGCCGCuAUGgGUCg -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 4166 | 0.66 | 0.744352 |
Target: 5'- uUCACCguugAACCGuCGGUcACCCAGa -3' miRNA: 3'- -AGUGGacuaUUGGCcGCUA-UGGGUCg -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 19062 | 0.66 | 0.733298 |
Target: 5'- aCGCCgc--AACCGGUgugGAUGCCguGCc -3' miRNA: 3'- aGUGGacuaUUGGCCG---CUAUGGguCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 5575 | 0.66 | 0.729959 |
Target: 5'- cUACCUccucGGUGAacauguaCGGCGGgguacggcgguguuUACCCGGCa -3' miRNA: 3'- aGUGGA----CUAUUg------GCCGCU--------------AUGGGUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 8902 | 0.66 | 0.722132 |
Target: 5'- cCAUCgggcgGGUGACCGGC----UCCGGCg -3' miRNA: 3'- aGUGGa----CUAUUGGCCGcuauGGGUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 21082 | 0.66 | 0.699515 |
Target: 5'- -gGCCaguuuUGAgaaaAACCGGCucGGUacuGCCCAGCa -3' miRNA: 3'- agUGG-----ACUa---UUGGCCG--CUA---UGGGUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 10570 | 0.66 | 0.697236 |
Target: 5'- cUCGCCgucugugUCGGCgGAUGCCCuGCa -3' miRNA: 3'- -AGUGGacuauu-GGCCG-CUAUGGGuCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 21438 | 0.67 | 0.64888 |
Target: 5'- cCAUCggcaucauuGCCGGCGGUGCaCUGGCg -3' miRNA: 3'- aGUGGacuau----UGGCCGCUAUG-GGUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 21575 | 0.67 | 0.641926 |
Target: 5'- cCGCgaUGAUGgcauugauGCCGGUGAUAaCCGGCc -3' miRNA: 3'- aGUGg-ACUAU--------UGGCCGCUAUgGGUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 20434 | 0.67 | 0.641926 |
Target: 5'- -----cGAUGGCCGGUguuaagcuacuGAUGCCCGGUu -3' miRNA: 3'- aguggaCUAUUGGCCG-----------CUAUGGGUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 22244 | 0.69 | 0.561169 |
Target: 5'- gCAuCCUG-UAACUGGCGGUcaagcugugucCCCGGCg -3' miRNA: 3'- aGU-GGACuAUUGGCCGCUAu----------GGGUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 1683 | 0.69 | 0.561169 |
Target: 5'- cCACCggccaccACCGGCGGUGCC-AGUa -3' miRNA: 3'- aGUGGacuau--UGGCCGCUAUGGgUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 3542 | 0.69 | 0.549808 |
Target: 5'- gUCACC-GAUGAcCCGGCaa-GCCuCGGCa -3' miRNA: 3'- -AGUGGaCUAUU-GGCCGcuaUGG-GUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 5421 | 0.7 | 0.494241 |
Target: 5'- gCGUCUGGauACCGGCGAUAUugCCGGUg -3' miRNA: 3'- aGUGGACUauUGGCCGCUAUG--GGUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 5945 | 0.7 | 0.483427 |
Target: 5'- aUCACCUGAcacUGcACCGGCGuccaccGCCCGa- -3' miRNA: 3'- -AGUGGACU---AU-UGGCCGCua----UGGGUcg -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 8080 | 0.7 | 0.462151 |
Target: 5'- gUCugCUGAccGCUGGCGAcguuguucgcccUGUCCAGCg -3' miRNA: 3'- -AGugGACUauUGGCCGCU------------AUGGGUCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 10995 | 0.71 | 0.431192 |
Target: 5'- -aGCCaGAguAUCGGCGAUAUCCuGCg -3' miRNA: 3'- agUGGaCUauUGGCCGCUAUGGGuCG- -5' |
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7112 | 3' | -52.6 | NC_001895.1 | + | 8233 | 0.71 | 0.41124 |
Target: 5'- -uGCCUGAUGcugugcGCCGGU--UGCaCCAGCg -3' miRNA: 3'- agUGGACUAU------UGGCCGcuAUG-GGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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