miRNA: 3'- aGUGGaCUAU---------------UGGCCGCUAUgGGUCG- -5'
miRNA: 3'- -AGUGGacu--AUUGGCCGCua--UGGG-UCG- -5'
miRNA: 3'- aGUGGacuau-UGGCCGCUAUGGgUCG- -5'
miRNA: 3'- -AGUG-GACU---AUUGGCCGcuaUGGGUCG- -5'
miRNA: 3'- -AGUGGACUAUUGGCCGCUAUGGGUCG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7112 | 3' | -52.6 | NC_001895.1 | + | 1757 | 0.72 | 0.386177 |
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Target: 5'- gCGCCgGAUGcgugguacuggcaccGCCGGUGGUGgCCGGUg -3' miRNA: 3'- aGUGGaCUAU---------------UGGCCGCUAUgGGUCG- -5' |
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| 7112 | 3' | -52.6 | NC_001895.1 | + | 5977 | 0.72 | 0.363954 |
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Target: 5'- uUCACCgucacUGACCGGCGugccGCCCuGGCg -3' miRNA: 3'- -AGUGGacu--AUUGGCCGCua--UGGG-UCG- -5' |
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| 7112 | 3' | -52.6 | NC_001895.1 | + | 4311 | 0.73 | 0.312369 |
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Target: 5'- cUACCgacacuGCCGGUGGUACCgAGCg -3' miRNA: 3'- aGUGGacuau-UGGCCGCUAUGGgUCG- -5' |
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| 7112 | 3' | -52.6 | NC_001895.1 | + | 7463 | 0.77 | 0.191302 |
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Target: 5'- cUCACgCUGAccgUAACCGGC---ACCCGGCa -3' miRNA: 3'- -AGUG-GACU---AUUGGCCGcuaUGGGUCG- -5' |
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| 7112 | 3' | -52.6 | NC_001895.1 | + | 6977 | 1.14 | 0.000469 |
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Target: 5'- aUCACCUGAUAACCGGCGAUACCCAGCa -3' miRNA: 3'- -AGUGGACUAUUGGCCGCUAUGGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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