miRNA: 3'- aGUGGacuaUUGGCCG---CUAUGGguCG- -5'
miRNA: 3'- agugGACUAU---UGGCCGCUAugGGUCG- -5'
miRNA: 3'- agugGACu-AUUGGCCgCUaUG-GGUCG- -5'
miRNA: 3'- -AGUGG--ACUa--UUGGCCGCuAUGgGUCg -5'
miRNA: 3'- -AGUGGacuaUUGGCcGCUA-UGGGUCg -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7112 | 3' | -52.6 | NC_001895.1 | + | 19062 | 0.66 | 0.733298 |
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Target: 5'- aCGCCgc--AACCGGUgugGAUGCCguGCc -3' miRNA: 3'- aGUGGacuaUUGGCCG---CUAUGGguCG- -5' |
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| 7112 | 3' | -52.6 | NC_001895.1 | + | 9263 | 0.66 | 0.744352 |
|
Target: 5'- ---aCUGAUGcacACUGGCGAUuuUCGGCg -3' miRNA: 3'- agugGACUAU---UGGCCGCUAugGGUCG- -5' |
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| 7112 | 3' | -52.6 | NC_001895.1 | + | 2128 | 0.66 | 0.744352 |
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Target: 5'- ---aCUGuuUAACCGGgGAcGCgCCAGCg -3' miRNA: 3'- agugGACu-AUUGGCCgCUaUG-GGUCG- -5' |
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| 7112 | 3' | -52.6 | NC_001895.1 | + | 11077 | 0.66 | 0.744352 |
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Target: 5'- aUCACCcgUGAgccGACCGGUG-UGCgCAGg -3' miRNA: 3'- -AGUGG--ACUa--UUGGCCGCuAUGgGUCg -5' |
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| 7112 | 3' | -52.6 | NC_001895.1 | + | 4166 | 0.66 | 0.744352 |
|
Target: 5'- uUCACCguugAACCGuCGGUcACCCAGa -3' miRNA: 3'- -AGUGGacuaUUGGCcGCUA-UGGGUCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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